- Catalog numberSMC-233D-A700
- Product nameMouse Anti-Human GRP170 Monoclonal IgG2aK, Clone 6G7-2H5
- Size100 µg
- PriceAsk For Price
- LabelATTO 700
- Clone6G7-2H5
- ImmunogenRaised against a synthetic peptide of human GRP170
- Antibody s full descriptionMouse Anti-Human GRP170 Monoclonal IgG2a Antibody, Clone: 6G7-2H5: ATTO 700
- Antibody s categoryMonoclonal Antibodies
- Antibody s other nameORP150 Antibody, HSP12A Antibody, Hypoxia up regulated 1 Antibody, Orp150 Antibody, Glucose regulated Antibody 170 Antibody, 150kDa oxygen regulated Antibody Antibody
- Raised inMouse
- IsotypeIgG2a
- Antibody s targetGRP170
- Primary research fieldsCancer, Heat Shock, Neuroscience, Neurodegeneration, Epigenetics, Trafficking, cardiovascular System
- BrandnameNone
- Antibodies applicationsWB, ICC/IF
- Antibody s reactivityHuman
- Antibody s dilutionsWB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
- PurityProtein G Purified
- Antibody buffer for storagePBS pH7.4, 50% glycerol, 0.1% sodium azide
- Antibody s concentration1 mg/ml
- Antibody s specificityDetects ~170kDa.
- Storage recommendations-20ºC
- Shipping recommendationsBlue Ice or 4ºC
- Antibody certificate of analysis1 µg/ml of SMC-233 was sufficient for detection of GRP170 in 20 µg of HEK293 lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
- Antibody in cellEndoplasmic Reticulum , Endoplasmic Reticulum Lumen
- Tissue specificitySee included datasheet or contact our support service
- Scientific contextGRP170, also known as ORP150, is the largest member of glucose-regulated Antibodys, and acts as a human chaperone Antibody. It is thought to play an important role in Antibody folding and secretion in the ER. Suppression of the Antibody is associated with accelerated apoptosis, therefore having an important cryoprotective role in hypoxia-induced cellular pertubation. This cryopotective role has led to an anti-tumor immune response, which will hopefully lead to therapeutic immunizations against cancers (1). GRP170 has also been shown to bind with dendritic cells and provide the danger signals to induce anti-tumor immune responses (2).
- Bibliography1. Wang H., et al. (2014) Front Oncol. 4: 377. 2. Manjili M.H., et al. (2006) Immun. Cell Biol. 84: 203-208.
- Released date2-Jul-2015
- Tested applicationsTo be tested
- Tested reactivityTo be tested
- NCBI numberNP_006389.3
- Gene number10525
- Protein numberQ9Y4L1
- Antibody s datasheetContact our support service
- Representative figure link
- Representative figure legendImmunocytochemistry/Immunofluorescence analysis using Mouse Anti-GRP170 Monoclonal Antibody, Clone 6G7-2H5 (SMC-233). Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-GRP170 Monoclonal Antibody (SMC-233) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm, Endoplasmic Reticulum. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) GRP170 Antibody (D) Composite. Western Blot analysis of Human HEK-293 cell lysate showing detection of ~170 kDa GRP170 protein using Mouse Anti-GRP170 Monoclonal Antibody, Clone 6G7-2H5 (SMC-233). Lane 1: Molecular Weight Ladder (MW). Lane 2: HEK-293 cell lysate. Load: 20 µg. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-GRP170 Monoclonal Antibody (SMC-233) at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:100 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~170 kDa. Mouse Anti-GRP170 Antibody [6G7-2H5] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Neuroblastoma cell line SK-N-BE (SMC-233) Mouse Anti-GRP170 Antibody [6G7-2H5] used in Western Blot (WB) on Human HEK-293 cell lysate (SMC-233)
- Warning informationNon-hazardous
- Country of productionCanada
- Total weight kg1.4
- Net weight g0.1
- Stock availabilityIn Stock
- Other related products
- Gene targetGRP170 Monoclonal IgG2aK 6G7-2H5
- Identity:HGNC:16931
- Gene:HYOU1
- Long gene name:hypoxia up-regulated 1
- Synonyms:ORP150HSP12AGrp170
- Discovery year:2002-05-27
- Entrez gene record:10525
- Pubmed identification:9020069, 10037731
- RefSeq identity:NM_006389
- Classification:Heat shock 70kDa proteins
- VEGA ID:OTTHUMG00000166354
- Gene symbolHYOU1
- Hostmouse
- isotype filter
- IgG2a
- Short nameMouse Anti- GRP170 Monoclonal IgG2aK, Clone 6G7-2H5
- label filter
- ATTO 700
- Clone name6G7-2H5
- technique filter
- anti-Human
- anti-
- Mouse
- anti
- Techniqueanti-Human, anti-, Mouse, anti, antibody to, antibodies, antibodies against human proteins, Monoclonals or monoclonal antibodies, mouses
- Alternative techniquemurine, antibodies