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Mouse Anti-Human FKBP52 Monoclonal IgG, Clone Hi52C

  • Catalog number
    SMC-139D-A488
  • Product name
    Mouse Anti-Human FKBP52 Monoclonal IgG, Clone Hi52C
  • Size
    100 µg
  • Label
    ATTO 488
  • Clone
    Hi52C
  • Immunogen
    Synthetic peptide corresponding to the residues of human FKBP52
  • Antibody s full description
    Mouse Anti-Human FKBP52 Monoclonal IgG Antibody, Clone: Hi52C: ATTO 488
  • Antibody s category
    Monoclonal Antibodies
  • Antibody s other name
    FK506 binding protein 4 Antibody, FKBP4 Antibody, FKBP59 Antibody, HBI Antibody, HSP56 Antibody, p52 Antibody, p59 Antibody, PPIase Antibody, Rotamase Antibody, T cell FK506 binding protein Antibody
  • Raised in
    Mouse
  • Isotype
    IgG, IgG
  • Antibody s target
    FKBP52
  • Primary research fields
    Cancer, Heat Shock, Cell Signaling, Trafficking
  • Brandname
    None
  • Antibodies applications
    WB, IHC, ICC/IF, IP
  • Antibody s reactivity
    Human, Mouse, Rat, Dog, Hamster
  • Antibody s dilutions
    WB (1:2000), IHC (1:250), ICC/IF (1:1000), IP (5µg); optimal dilutions for assays should be determined by the user.
  • Purity
    Protein G Purified
  • Antibody buffer for storage
    PBS, 50% glycerol, 0.09% sodium azide
  • Antibody s concentration
    1 mg/ml
  • Antibody s specificity
    Detects ~52kDa. Heavy chain migrates close to FKBP52 on SDS PAGE.
  • Storage recommendations
    -20ºC
  • Shipping recommendations
    Blue Ice or 4ºC
  • Antibody certificate of analysis
    0.5 µg/ml was sufficient for detection of FKBP52 in 20 µg total protein using WB by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary.
  • Antibody in cell
    Cytoplasm , Nucleus
  • Tissue specificity
    See included datasheet or contact our support service
  • Scientific context
    HSP90 is crucial to cellular signaling by its regulation of the folding, activity, and stability of a wide range of client proteins. These client protein complexes may also contain one or more cochaperones (1). One class of HSP90-binding cochaperone is composed of proteins with a characteristic tetratricopeptide repeat (TPR) domain that forms an HSP90 binding site. Among the TPR cochaperones of HSP90 are Hop/Sti1, protein phosphatase PP5, and members of both the FK506- and cyclosporin A-binding families of immunophilins (2). FK506-binding protein 51 (FKBP51) and FKBP52 are large molecular weight immunophilins that are part of the mature glucocorticoid receptor (GR) heterocomplex (3). The N terminal domain of each protein binds FK506 and has peptidyl-prolyl isomerase (PPIase) activity that converts prolyl peptide bonds within target proteins from cis- to trans- proline. The C-terminal domains contain the TPR repeats involved in protein-protein interactions with the HSP90 (4). Although FKBP52 and FKBP51 share ~75% sequence similarity, they affect hormone binding by glucocorticoid receptor in opposing manners and have different HSP90-binding characteristics (3). FK506 binding protein 51 kDa (FKBP51 or otherwise referred to as FKBP54) has been identified as a progestininducible gene. This protein is predominantly expressed in murine T cells but in humans, it is abundantly expressed in numerous tissues at levels many times higher than FKBP12. The FKBP51 gene is known to be induced by glucocorticoids (5).
  • Bibliography
    1. Cheung-Flynn J., Roberts P.J., Riggs D.L., and Smith D.F. (2003) J. Biol. Chem. 278(19): 17388-17394. 2. Davies T.H., Ning Y.N., and Sanchez E.R. (2002) J Biol. Chem. 277 (7): 4597-4600. 3. Wu, B. et al. (2004) Proc. Natl. Acad. Sci. USA. 101(22): 8348-8353. 4. Denny W.B., Prapapanich V., Smith D.F., and Scammell J.G. (2005) Endocrinology 146(7):3194-3201. 5. Cox M.B. et al. (2007) Molecular Endocrinology. Epub.
  • Tested applications
    To be tested
  • Tested reactivity
    To be tested
  • NCBI number
    NP_002005.1
  • Gene number
    2288
  • Protein number
    Q02790
  • Antibody s datasheet
    Contact our support service
  • Representative figure link
    Representative_figure_link
  • Representative figure legend
    Immunohistochemistry analysis using Mouse Anti-FKBP52 Monoclonal Antibody, Clone Hi52C (SMC-139). Tissue: prostate tissue (ductal epithelial cells). Species: Human. Primary Antibody: Mouse Anti-FKBP52 Monoclonal Antibody (SMC-139) at 1:1000. Courtesy of: David F. Smith, Mayo Clinic, USA. Immunohistochemistry analysis using Mouse Anti-FKBP52 Monoclonal Antibody, Clone Hi52C (SMC-139). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-FKBP52 Monoclonal Antibody (SMC-139) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Epidermis. Western Blot analysis of Rat Brain, Heart, Kidney, Liver, Pancreas, Skeletal muscle, Spleen, Testes, Thymus cell lysates showing detection of FKBP52 protein using Mouse Anti-FKBP52 Monoclonal Antibody, Clone Hi52C (SMC-139). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-FKBP52 Monoclonal Antibody (SMC-139) at 1.5 µg/mL for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT. Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-FKBP52 Monoclonal Antibody, Clone Hi52C (SMC-139). Tissue: MCF-7 cells (metastatic mammary gland/breast cell line). Species: Human. Primary Antibody: Mouse Anti-FKBP52 Monoclonal Antibody (SMC-139) at 1:1000. Secondary Antibody: APC Goat Anti-Mouse (red). Courtesy of: Tom Ratajczak, Univ. of W. Australia. Western Blot analysis of Human Cell lysates showing detection of FKBP52 protein using Mouse Anti-FKBP52 Monoclonal Antibody, Clone Hi52C (SMC-139). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-FKBP52 Monoclonal Antibody (SMC-139) at 1.5 µg/mL for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT. Mouse Anti-FKBP52 Antibody [Hi52C] used in Immunohistochemistry (IHC) on Human prostate tissue (ductal epithelial cells) (SMC-139) Mouse Anti-FKBP52 Antibody [Hi52C] used in Immunohistochemistry (IHC) on Mouse backskin (SMC-139) Mouse Anti-FKBP52 Antibody [Hi52C] used in Western Blot (WB) on Rat Brain, Heart, Kidney, Liver, Pancreas, Skeletal muscle, Spleen, Testes, Thymus cell lysates (SMC-139) Mouse Anti-FKBP52 Antibody [Hi52C] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human MCF-7 cells (metastatic mammary gland/breast cell line) (SMC-139) Mouse Anti-FKBP52 Antibody [Hi52C] used in Western Blot (WB) on Human Cell lysates (SMC-139)
  • Warning information
    Non-hazardous
  • Country of production
    Canada
  • Total weight kg
    1.4
  • Net weight g
    0.1
  • Stock availability
    In Stock
  • Other related products
    Other_related_products
  • Gene target
    FKBP52 Monoclonal Hi52C
    • Gene info
  • Gene symbol
    FKBP4
  • Host
    mouse
  • isotype filter
    • IgG
  • Short name
    Mouse Anti- FKBP52 Monoclonal IgG, Clone Hi52C
  • label filter
    • ATTO 488
  • Clone name
    Hi52C
  • technique filter
    • anti-Human
    • anti-
    • Mouse
    • anti
    • IgG
  • Technique
    anti-Human, anti-, Mouse, anti, IgG, antibody to, antibodies, antibodies against human proteins, IgGs, Monoclonals or monoclonal antibodies, mouses
  • Alternative technique
    murine, antibodies, immunoglobulins