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Mouse Anti-Human ERp57 (GRP58) Monoclonal IgG1, Clone Map.ERp57

  • Catalog number
    SMC-168B-RPE
  • Product name
    Mouse Anti-Human ERp57 (GRP58) Monoclonal IgG1, Clone Map.ERp57
  • Size
    200 µg
  • Label
    RPE
  • Clone
    Map.ERp57
  • Immunogen
    Human recombinant ERp57 (Grp58)
  • Antibody s full description
    Mouse Anti-Human ERp57 Monoclonal IgG1 Antibody, Clone: Map.ERp57: RPE
  • Antibody s category
    Monoclonal Antibodies
  • Antibody s other name
    ERp60 Antibody, ERp61 Antibody, Grp57 Antibody, Grp58 Antibody, P58 Antibody, PDIA3 Antibody, PI PLC Antibody, 58 kDa glucose regulated protein antibody, 58 kDa glucose-regulated protein antibody, 58 kDa microsomal protein antibody, Disulfide isomerase ER 60 antibody, Disulfide isomerase ER-60 antibody, Endoplasmic reticulum resident protein 57 antibody, Endoplasmic reticulum resident protein 60 antibody, ER p57 antibody, ER protein 57 antibody, ER protein 60 antibody, ERp 57 antibody, ERp57 antibody, Glucose Regulated Protein 58 Kd antibody, GRP 57 antibody, GRP 58 antibody, GRP57 antibody, HsT17083 antibody, p58 antibody, PDIA 3 antibody, PDIA3 antibody, PDIA3_HUMAN antibody, Phospholipase C alpha antibody, PI PLC antibody, Protein disulfide isomerase A3 antibody, Protein disulfide isomerase family A member 3 antibody, Protein disulfide-isomerase A3 antibody
  • Raised in
    Mouse
  • Isotype
    IgG1, IgG1
  • Antibody s target
    ERp57
  • Primary research fields
    Cell Signaling, Protein Trafficking
  • Brandname
    None
  • Antibodies applications
    WB, IHC, ICC/IF, IP
  • Antibody s reactivity
    Human, Mouse, Rat, Bovine, Dog, Guinea Pig (Cavia porcellus), Hamster, Monkey, Pig, Rabbit
  • Antibody s dilutions
    WB (1:2000), IHC (1:100), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
  • Purity
    Protein G Purified
  • Antibody buffer for storage
    PBS pH7.4, 50% glycerol, 0.09% sodium azide
  • Antibody s concentration
    1 mg/ml
  • Antibody s specificity
    Detects ~57kDa.
  • Storage recommendations
    -20ºC
  • Shipping recommendations
    Blue Ice or 4ºC
  • Antibody certificate of analysis
    0.5 µg/ml of SMC-168 was sufficient for detection of ERp57 in 10 µg of heat shock Heal Lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
  • Antibody in cell
    Endoplasmic Reticulum , Endoplasmic Reticulum Lumen , Melanosome
  • Tissue specificity
    See included datasheet or contact our support service
  • Scientific context
    ERp57, also known as Glucose Regulated Protein 58 (Grp58), Hormone-Induced Protein-70 (HIP-70) and microsomal Carnitine Palmitoyltransferase, is a member of the protein disulfide isomerase family, containing two canonical CXHC tetrapeptide active site motifs (1-5). It has quite a few diverse roles. It functions as an accessory oxidoreductase involved in disulfide bond formation. In the ER, ERp57 interacts with membrane bound calnexin and soluble calreticulin (lectin chaperones) via their praline rich P-domain arms. Lectin chaperones bind nascent non-native glycoproteins, and position ERp57 to act upon the immature or misfolded glycoproteins that possess mono-glycosylated side chains. ERp57 deletion impairs posttranslational phases of influenza hema-glutinin folding, and causes accelerated release of MHC-I molecules, resulting in the coupling of sub-optimal peptides and reduced expression and stability on the cell surface (6). ERp57 also contains two thioredoxin active-site sequences, CGHC and an estrogen-binding domain. ERp57 is induced by both estrogen and leuteinizing-hormone-releasing hormone in the hippocampus (7).
  • Bibliography
    1. Herbert D.N. and Molinari M. (2007) Physiol Rev. 87: 1377-1408. 2. Williams D.B. (2005) J Cell Sci. 119: 615-623 3. Maattanen P., et al. (2006) Biochem Cell Biol. 84: 881-889. 4. Oliver J.D., et al. (1999) Mol Bio Cell. 10: 2573-2582. 5. Oliver J.D., et al. (1997) Science 275: 86-88. 6. Solda T., et al. (2006) J Biol Chem 281: 6219-6226. 7. Kimura T., et al. (2005) Biochem Biophys Research Communications. 331 (1): 224-230. 8. Chen, G., et al. (2002) Clin Cancer Res 8(7): 2298-2305. 9. Tan, P., et al. F. (2002) J Immunol 168(4): 1950-1960.
  • Released date
    1-Apr-2009
  • PubMed number
    25767741, 25767741, 23843619
  • Tested applications
    Western Blot, Immunocytochemistry/Immunofluorescence, Immunoprecipitation
  • Tested reactivity
    Human, Human, Human
  • NCBI number
    NP_005304.3
  • Gene number
    2923
  • Protein number
    P30101
  • Antibody s datasheet
    Contact our support service
  • Representative figure link
    Representative_figure_link
  • Representative figure legend
    Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Erp57 (Grp58) Monoclonal Antibody, Clone Map.ERP57 (SMC-168). Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Mouse Anti-Erp57 (Grp58) Monoclonal Antibody (SMC-168) at 1:100 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Endoplasmic reticulum lumen. Melanosome. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-Erp57 (Grp58) Antibody. (C) Composite. Immunohistochemistry analysis using Mouse Anti-Erp57 Monoclonal Antibody, Clone Map.ERP57 (SMC-168). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Erp57 Monoclonal Antibody (SMC-168) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Epidermis and Hair Follicles. Western Blot analysis of Human cell lysates showing detection of Erp57 protein using Mouse Anti-Erp57 Monoclonal Antibody, Clone Map.ERP57 (SMC-168). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Erp57 Monoclonal Antibody (SMC-168) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT. Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Erp57 Monoclonal Antibody, Clone Map.ERP57 (SMC-168). Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20°C. Primary Antibody: Mouse Anti-Erp57 Monoclonal Antibody (SMC-168) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Cytoplasmic and perinuclear staining. Mouse Anti-Erp57 (Grp58) Antibody [Map.ERP57] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Heat Shocked HeLa Cells (SMC-168) Mouse Anti-Erp57 Antibody [Map.ERP57] used in Immunohistochemistry (IHC) on Mouse backskin (SMC-168) Mouse Anti-Erp57 Antibody [Map.ERP57] used in Western Blot (WB) on Human cell lysates (SMC-168) Mouse Anti-Erp57 Antibody [Map.ERP57] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human HaCaT cells (SMC-168)
  • Warning information
    Non-hazardous
  • Country of production
    Canada
  • Total weight kg
    1.4
  • Net weight g
    0.2
  • Stock availability
    In Stock
  • Other related products
    Other_related_products
  • Gene target
    ERp57 GRP58 Monoclonal IgG1 Map ERp57
    • Gene info
  • Gene symbol
    PDIA3
  • Host
    mouse
  • isotype filter
    • IgG1
  • Short name
    Mouse Anti- ERp57 (GRP58) Monoclonal IgG1, Clone Map ERp57
  • label filter
    • RPE
  • Clone name
    Map
  • technique filter
    • anti-Human
    • anti-
    • Mouse
    • anti
  • Technique
    anti-Human, anti-, Mouse, anti, antibody to, antibodies, antibodies against human proteins, Monoclonals or monoclonal antibodies, mouses
  • Alternative technique
    murine, antibodies