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Mouse Anti-Human Ankyrin-B Monoclonal IgG2A, Clone S105-17

  • Catalog number
    SMC-414D-A390
  • Product name
    Mouse Anti-Human Ankyrin-B Monoclonal IgG2A, Clone S105-17
  • Size
    100 µg
  • Label
    ATTO 390
  • Clone
    S105-17
  • Immunogen
    Synthetic peptide amino acids 203-496 of human Ankyrin-B
  • Antibody s full description
    Mouse Anti-Human Ankyrin B Monoclonal IgG2A Antibody, Clone: S105-17: ATTO 390
  • Antibody s category
    Monoclonal Antibodies
  • Antibody s other name
    ANK-2 Antibody, ANK2 Antibody, Ankyrin 2 Antibody, Ankyrin-2 Antibody, Ankyrin nonerythroid Antibody, Brain ankyrin Antibody, non-erythroid ankyrin Antibody, Ankyrin-B Antibody
  • Raised in
    Mouse
  • Isotype
    IgG2A, IgG2a
  • Antibody s target
    Ankyrin B
  • Primary research fields
    Neuroscience, Cell Structure, Membrane Markers, Cell Signaling, Scaffolds
  • Brandname
    None
  • Antibodies applications
    WB, IHC, ICC/IF
  • Antibody s reactivity
    Human, Mouse, Rat
  • Antibody s dilutions
    WB (1:1000); optimal dilutions for assays should be determined by the user.
  • Purity
    Protein G Purified
  • Antibody buffer for storage
    PBS pH7.4, 50% glycerol, 0.09% sodium azide
  • Antibody s concentration
    1 mg/ml
  • Antibody s specificity
    Detects ~>200kDa. No cross-reactivity against Ankyrin-G.
  • Storage recommendations
    -20ºC
  • Shipping recommendations
    Blue Ice or 4ºC
  • Antibody certificate of analysis
    1 µg/ml of SMC-414 was sufficient for detection of Ankyrin-B in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
  • Antibody in cell
    Cytoplasm , Cytoskeleton , Myofibril , Sarcomere , M line , Apical Cell Membrane , Cell Membrane
  • Tissue specificity
    See included datasheet or contact our support service
  • Scientific context
    Ankyrins are a family of adaptor proteins that mediate the attachment of integral membrane proteins to the spectrin-actin based membrane skeleton (1). Ankyrins have binding sites for the beta subunit of spectrin and at least 12 families of integral membrane proteins. This linkage is required to maintain the integrity of the plasma membranes and to anchor specific ion channels, ion exchangers and ion transporters in the plasma membrane.
  • Bibliography
    1. Bennett V., Baines A.J. (2001) Physiol. Rev. 81 (3): 1353–92.
  • Released date
    3-Apr-2013
  • Tested applications
    To be tested
  • Tested reactivity
    To be tested
  • NCBI number
    NP_001120965.1
  • Gene number
    287
  • Protein number
    Q01484
  • Antibody s datasheet
    Contact our support service
  • Representative figure link
    Representative_figure_link
  • Representative figure legend
    Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Ankyrin-B Monoclonal Antibody, Clone S105-17 (SMC-414). Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Ankyrin-B Monoclonal Antibody (SMC-414) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Cytoplasm, Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) Ankyrin-B Antibody (D) Composite. Western Blot analysis of Rat brain membrane lysate showing detection of Ankyrin B protein using Mouse Anti-Ankyrin B Monoclonal Antibody, Clone S105-17 (SMC-414). Primary Antibody: Mouse Anti-Ankyrin B Monoclonal Antibody (SMC-414) at 1:1000. Mouse Anti-Ankyrin-B Antibody [S105-17] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Neuroblastoma cell line SK-N-BE (SMC-414) Mouse Anti-Ankyrin B Antibody [S105-17] used in Western Blot (WB) on Rat brain membrane lysate (SMC-414)
  • Warning information
    Non-hazardous
  • Country of production
    Canada
  • Total weight kg
    1.4
  • Net weight g
    0.1
  • Stock availability
    In Stock
  • Other related products
    Other_related_products
  • Gene target
    Ankyrin-B Monoclonal IgG2A S105-17
    • Gene info
    • Identity:HGNC:37599
    • Gene:PIRC95
    • Long gene name:piwi-interacting RNA cluster 95
    • Discovery year:2009-11-05
    • Entrez gene record:100313935
    • Pubmed identification:17881367
    • Classification:Piwi-interacting RNA clusters
    Gene info
    • Identity:HGNC:37595
    • Gene:PIRC91
    • Long gene name:piwi-interacting RNA cluster 91
    • Discovery year:2009-11-05
    • Entrez gene record:100313900
    • Pubmed identification:17881367
    • Classification:Piwi-interacting RNA clusters
    Gene info
    • Identity:HGNC:37594
    • Gene:PIRC90
    • Long gene name:piwi-interacting RNA cluster 90
    • Discovery year:2009-11-05
    • Entrez gene record:100313798
    • Pubmed identification:17881367
    • Classification:Piwi-interacting RNA clusters
    Gene info
    Gene info
    • Identity:HGNC:55105
    • Gene:IGHV3OR16-17
    • Long gene name:immunoglobulin heavy variable 3/OR16-17 (non-functional)
    • Synonyms:IGHV3/OR16-17
    • Discovery year:2020-06-26
    • Entrez gene record:390714
    • Classification:Immunoglobulin heavy (IGH) orphons
    Gene info
    • Identity:HGNC:37596
    • Gene:PIRC92
    • Long gene name:piwi-interacting RNA cluster 92
    • Discovery year:2009-11-05
    • Entrez gene record:100313920
    • Pubmed identification:17881367
    • Classification:Piwi-interacting RNA clusters
    Gene info
    • Identity:HGNC:37597
    • Gene:PIRC93
    • Long gene name:piwi-interacting RNA cluster 93
    • Discovery year:2009-11-05
    • Entrez gene record:100313934
    • Pubmed identification:17881367
    • Classification:Piwi-interacting RNA clusters
    Gene info
  • Gene symbol
    PIRC95, PIRC91, PIRC90, SNORD115-17, IGHV3OR16-17, PIRC92, PIRC93, SNORD116-17
  • Host
    mouse
  • isotype filter
    • IgG2A
  • Short name
    Mouse Anti- Ankyrin-B Monoclonal IgG2A, Clone S105-17
  • label filter
    • ATTO 390
  • Clone name
    S105-17
  • technique filter
    • anti-Human
    • anti-
    • Mouse
    • anti
  • Technique
    anti-Human, anti-, Mouse, anti, antibody to, antibodies, antibodies against human proteins, Monoclonals or monoclonal antibodies, mouses
  • Alternative technique
    murine, antibodies