- Catalog numberSMC-159B-A594
- Product nameMouse Anti-Human Alpha B Crystallin Monoclonal IgG1, Clone 1A7.D5
- Size200 µg
- PriceAsk For Price
- LabelATTO 594
- Clone1A7.D5
- ImmunogenNative Alpha B Crystallin
- Antibody s full descriptionMouse Anti-Human Alpha B Crystallin Monoclonal IgG1 Antibody, Clone: 1A7.D5: ATTO 594
- Antibody s categoryMonoclonal Antibodies
- Antibody s other nameAACRYA Antibody, CRYA2 Antibody, CRYAB Antibody, CTPP2 Antibody, HSPB5 Antibody, NY Ren 27 antigen Antibody, alpha crystallin BAntibody
- Raised inMouse
- IsotypeIgG1, IgG1
- Antibody s targetAlpha B Crystallin
- Primary research fieldsCancer, Heat Shock, Cell Signaling, Trafficking, Chaperones, Neuroscience
- BrandnameNone
- Antibodies applicationsWB, ELISA, ICC/IF
- Antibody s reactivityHuman, Bovine, Pig, Rat
- Antibody s dilutionsWB (1:2000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
- PurityProtein G Purified
- Antibody buffer for storagePBS pH7.2, 50% glycerol, 0.09% sodium azide
- Antibody s concentration1 mg/ml
- Antibody s specificityDetects ~20kDa (Predicted mol. weight is ~21kDa). Does not cross-react with αA-crystallin, βL-crystallin, βH-crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins.
- Storage recommendations-20ºC
- Shipping recommendationsBlue Ice or 4ºC
- Antibody certificate of analysis0.5 µg/ml of SMC-159 was sufficient for detection of 50 ng purified alpha B crystalline by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
- Antibody in cellCytoplasm , Nucleus
- Tissue specificitySee included datasheet or contact our support service
- Scientific contextThe alpha-crystallins are major water-soluble lens structural proteins of the vertebrate eye that are related to the small heat shock protein family. The alpha-crystallins possess structural and functional similarities with HSP25 and HSP27 (1). Mammalian lens cystallins are divided into alpha, beta and gamma families. Alpha and beta families are further divided into acidic and basic groups (Alpha-A and Alpha-B respectively). In the lens, alpha-crystallin primarily functions to maintain proper refractive index, however it can also function as a molecular chaperone that binds to the denatured proteins, keeping them in solution and thereby maintaining the translucency of the lens. When cellular stress occurs, alpha-crystallin enters its’ phosphorylated state and may serve a structural control function and play a role in protein maintenance (2). In addition to their interaction with proteins, alpha-crystallins also interact with native molecules such as membrane proteins, Golgi matrix protein, structural proteins, nuclear proteins and DNA (3, 4, 5, 6, and 7). Two other functions are an autokinase activity and participation in the intracellular architecture, and it has also been proven that both alpha-A and B prevent apoptosis by inhibiting caspases (8). Specifically, alpha-B cystallin is found in many cells and organs outside the lens, and alpha B is overexpressed in several neurological disorders and in cell lines under stress conditions (9).
- Bibliography1. Merck K.B. et al. (1993) J Biol Chem. 268: 1046-1052. 2. Horwitz J. (1992) Proc Natl Acad Sci USA. 89(21): 10449-10453. 3. Cobb B.A. and Petrash J.M. (2002) Biochemistry. 41: 483-490 4. Horwitz J. (2003) Exp Eye Res. 76: 145-153. 5. Bullard B. et al. (2004) J Biol Chem. 279: 7917-7924. 6. Gangalum R.K., Schibler M.J. and Bhat S.P. (2004) J Biol Chem. 279: 43374.43377. 7. Maddala R. and Rao V.P. (2005) Exp Cell Res. 306: 203-215. 8. Yaung J., et al. (2007) Molecular Vision 13: 566-577. 9. Head M.W. et al. (2000) Neuropathol Appl Neurobiol. 26: 304-312. 10. Banduseela V.C., et al. (2009) Physiol Genomics. 39(3): 141-159.
- Released date1-Aug-2008
- PubMed number24705179, 22010006, 21320889, 19706692
- Tested applicationsWestern Blot, Western Blot, Western Blot, Western Blot
- Tested reactivityRat, Pig, Rat, Pig
- NCBI numberNP_001876.1
- Gene number1410
- Protein numberP02511
- Antibody s datasheetContact our support service
- Representative figure link
- Representative figure legendImmunocytochemistry/Immunofluorescence analysis using Mouse Anti-Alpha B Crystallin Monoclonal Antibody, Clone 1A7.D5 (SMC-159). Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Alpha B Crystallin Monoclonal Antibody (SMC-159) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Membrane. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) Alpha B Crystallin Antibody (D) Composite. Western Blot analysis of Bovine cell lysates showing detection of Alpha B Crystallin protein using Mouse Anti-Alpha B Crystallin Monoclonal Antibody, Clone 1A7.D5 (SMC-159). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Alpha B Crystallin Monoclonal Antibody (SMC-159) at 1:50 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT. Mouse Anti-Alpha B Crystallin Antibody [1A7.D5] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Neuroblastoma cell line SK-N-BE (SMC-159) Mouse Anti-Alpha B Crystallin Antibody [1A7.D5] used in Western Blot (WB) on Bovine cell lysates (SMC-159)
- Warning informationNon-hazardous
- Country of productionCanada
- Total weight kg1.4
- Net weight g0.2
- Stock availabilityIn Stock
- Other related products
- Gene targetAlpha B Crystallin Monoclonal IgG1 1A7 D5
- Hostmouse
- isotype filter
- IgG1
- Short nameMouse Anti- Alpha B Crystallin Monoclonal IgG1, Clone 1A7 D5
- label filter
- ATTO 594
- Clone name1A7
- technique filter
- anti-Human
- anti-
- Mouse
- anti
- Techniqueanti-Human, anti-, Mouse, anti, antibody to, antibodies, antibodies against human proteins, Monoclonals or monoclonal antibodies, mouses
- Alternative techniquemurine, antibodies