- Catalog numberSMC-106B-A700
- Product nameMouse Anti-Chicken HSP70/HSC70 Monoclonal IgG2a, Clone BB70
- Size200 µg
- PriceAsk For Price
- LabelATTO 700
- CloneBB70
- ImmunogenChicken HSP70/HSP90 complex
- Antibody s full descriptionMouse Anti-Chicken HSP70/HSC70 Monoclonal IgG2a Antibody, Clone: BB70: ATTO 700
- Antibody s categoryMonoclonal Antibodies
- Antibody s other nameHSC54 Antibody, HSC70 Antibody, HSC71 Antibody, HSP70 1 Antibody, HSP701/HSP70 2 Antibody, HSP70.1 Antibody, HSP71 Antibody, HSP72 Antibody, HSP73 Antibody, HSPA1 Antibody, HSPA10 Antibody, HSPA1A Antibody, HSPA1B Antibody, LAP1 Antibody, NIP71 Antibody
- Raised inMouse
- IsotypeIgG2a, IgG2a
- Antibody s targetHSP70/HSC70
- Primary research fieldsCancer, Heat Shock
- BrandnameNone
- Antibodies applicationsWB, IHC, IP
- Antibody s reactivityHuman, Mouse, Rat, Bovine, Sheep, Dog, Beluga, Fish, Guinea Pig (Cavia porcellus), Pig, Hamster, Rabbit, Chicken, African clawed frog (Xenopus laevis), Fruit Fly (Drosophila melanogaster), Yeast
- Antibody s dilutionsWB (1:1000), IHC (1:200), ICC/IF (1:200); optimal dilutions for assays should be determined by the user.
- PurityProtein G Purified
- Antibody buffer for storagePBS pH7.2, 50% glycerol, 0.09% sodium azide
- Antibody s concentration1 mg/ml
- Antibody s specificityDetects ~72 (HSP) and ~73kDa (HSC).
- Storage recommendations-20ºC
- Shipping recommendationsBlue Ice or 4ºC
- Antibody certificate of analysis1 µg/ml of SMC-106 was sufficient for detection of HSP70 and HSC70 in 20 µg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
- Antibody in cellCytoplasm
- Tissue specificitySee included datasheet or contact our support service
- Scientific context
- Bibliography1. Zho J. (1998) Cell 94 : 471-480. 2. Boorstein W. R., Ziegelhoffer T. & Craig E. A. (1993) J. Mol. Evol.38 (1): 1-17. 3. Rothman J. (1989) Cell 59: 591 -601. 4. DeLuca-Flaherty et al. (1990) Cell 62: 875-887. 5. Bork P., Sander C. & Valencia A. (1992) Proc. Nat Acad. Sci. USA 89: 7290-7294. 6. Fink A.L. (1999) Physiol. Rev. 79: 425-449. 7. Smith D.F., et al, (1993) Mol. Cell. Biol. 13(2): 869-876. 8. Prapapanich V., et al. (1996) Mol. Cell. Biol. 16(11):6200-6207. 9. Fernandez-Funez et al., (2000) Nature 408(6808): 101-106.
- Released date1-Jun-2007
- PubMed number24934503, 24548207, 24548239, 24239008
- Tested applicationsWestern Blot, Western Blot, Western Blot, Protein Binding Assay
- Tested reactivityHuman, Human, Human, Human
- NCBI numberNP_001006686.1
- Gene number423504
- Protein numberP08106
- Antibody s datasheetContact our support service
- Representative figure link
- Representative figure legendImmunohistochemistry analysis using Mouse Anti-Hsp70 Monoclonal Antibody, Clone BB70 (SMC-106). Tissue: inflamed colon. Species: Mouse. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody (SMC-106) at 1:10000 for 12 hours at 4°C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 µl for 2 minutes at RT. Localization: Inflammatory cells. Magnification: 40x. Immunohistochemistry analysis using Mouse Anti-Hsp70 Monoclonal Antibody, Clone BB70 (SMC-106). Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody (SMC-106) at 1:10000 for 12 hours at 4°C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 µl for 2 minutes at RT. Localization: Inflammatory cells. Magnification: 40x. Western Blot analysis of Bovine MDBK cell lysates showing detection of Hsp70 protein using Mouse Anti-Hsp70 Monoclonal Antibody, Clone BB70 (SMC-106). Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody (SMC-106) at 1:1000. Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Hsp70 Monoclonal Antibody, Clone BB70 (SMC-106). Tissue: hepatocyte nuclei. Species: Rat. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody (SMC-106) at 1:200. Courtesy of: G. Matic, University of Belgrade, Serbia. Western Blot analysis of Human HeLa cell lysates showing detection of Hsp70 protein using Mouse Anti-Hsp70 Monoclonal Antibody, Clone BB70 (SMC-106). Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody (SMC-106) at 1:1000. Secondary Antibody: HRP Goat Anti-Rat. Immunohistochemistry analysis using Mouse Anti-Hsp70 Monoclonal Antibody, Clone BB70 (SMC-106). Tissue: hepatocytes. Species: Rat. Fixation: Paraffin Embedded. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody (SMC-106) at 1:200. Courtesy of: G. Matic, University of Belgrade, Serbia. Mouse Anti-Hsp70 Antibody [BB70] used in Immunohistochemistry (IHC) on Mouse inflamed colon (SMC-106) Mouse Anti-Hsp70 Antibody [BB70] used in Immunohistochemistry (IHC) on Human colon carcinoma (SMC-106) Mouse Anti-Hsp70 Antibody [BB70] used in Western Blot (WB) on Bovine MDBK cell lysates (SMC-106) Mouse Anti-Hsp70 Antibody [BB70] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Rat hepatocyte nuclei (SMC-106) Mouse Anti-Hsp70 Antibody [BB70] used in Western Blot (WB) on Human HeLa cell lysates (SMC-106) Mouse Anti-Hsp70 Antibody [BB70] used in Immunohistochemistry (IHC) on Rat hepatocytes (SMC-106)
- Warning informationNon-hazardous
- Country of productionCanada
- Total weight kg1.4
- Net weight g0.2
- Stock availabilityIn Stock
- Other related products
- Gene target- HSP70/HSC70 Monoclonal IgG2a BB70
- Identity:HGNC:5233
- Gene:HSPA1B
- Long gene name:heat shock protein family A (Hsp70) member 1B
- Synonyms gene name:heat shock 70kD protein 1B, heat shock 70kDa protein 1B
- Synonyms:HSP70-2,
- Discovery year:1995-08-11
- Entrez gene record:3304
- Pubmed identification:1700760
- Classification:TIM23 complex, Heat shock 70kDa proteins
- VEGA ID:OTTHUMG00000031202
- Identity:HGNC:5232
- Gene:HSPA1A
- Long gene name:heat shock protein family A (Hsp70) member 1A
- Synonyms gene name:heat shock 70kD protein 1A, heat shock 70kDa protein 1A
- Synonyms:HSP70-1,
- Discovery year:2001-06-22
- Entrez gene record:3303
- Classification:TIM23 complex, Heat shock 70kDa proteins
- VEGA ID:OTTHUMG00000031201
- Identity:HGNC:29526
- Gene:HSPA14
- Long gene name:heat shock protein family A (Hsp70) member 14
- Synonyms gene name:heat shock 70kDa protein 14
- Synonyms:HSP70-4HSP70L1
- Discovery year:2004-04-20
- Entrez gene record:51182
- Pubmed identification:21208456
- RefSeq identity:NM_016299
- Classification:Heat shock 70kDa proteins
- Identity:HGNC:5234
- Gene:HSPA1L
- Long gene name:heat shock protein family A (Hsp70) member 1 like
- Synonyms gene name:heat shock 70kD protein-like 1, heat shock 70kDa protein 1-like
- Synonyms:HSP70-HOM, hum70t
- Discovery year:1989-05-12
- Entrez gene record:3305
- Pubmed identification:9685725, 9349405
- Classification:TIM23 complex, Heat shock 70kDa proteins
- VEGA ID:OTTHUMG00000031208
- Identity:HGNC:5241
- Gene:HSPA8
- Long gene name:heat shock protein family A (Hsp70) member 8
- Synonyms gene name:heat shock 70kD protein 8, heat shock 70kDa protein 8
- Synonyms:HSC71, HSC70HSP73
- Discovery year:1995-05-08
- Entrez gene record:3312
- Pubmed identification:8530083, 3037489
- Classification:Spliceosomal P complex, Heat shock 70kDa proteins, Spliceosomal C complex
- VEGA ID:OTTHUMG00000166030
- Gene symbolHSPA1B, HSPA1A, HSPA14, HSPA1L, HSPA8
- Hostmouse
- isotype filter
- IgG2a
- Short nameMouse Anti- HSP70/HSC70 Monoclonal IgG2a, Clone BB70
- label filter
- ATTO 700
- Clone nameBB70
- technique filter
- anti-
- Mouse
- anti
- Techniqueanti-, Mouse, anti, antibody to, antibodies, Monoclonals or monoclonal antibodies, mouses
- Alternative techniquemurine, antibodies