• Catalog number
    SMC-160B-A594
  • Product name
    Mouse Anti-Bovine Ubiquitin Monoclonal IgG2a Kappa, Clone 5B9-B3
  • Size
    200 µg
  • Label
    ATTO 594
  • Clone
    5B9-B3
  • Immunogen
    Native bovine ubiquitin, conjugated to KLH
  • Antibody s full description
    Mouse Anti-Bovine Ubiquitin Monoclonal IgG2a Kappa Antibody, Clone: 5B9-B3: ATTO 594
  • Antibody s category
    Monoclonal Antibodies
  • Antibody s other name
    Polyubiquitin B Antibody, RPS27A Antibody, UBA52 Antibody, UBB Antibody, UBC Antibody, ubiquitin B Antibody
  • Raised in
    Mouse
  • Isotype
    IgG2a Kappa, IgG2a
  • Antibody s target
    Ubiquitin
  • Primary research fields
    Cell Signaling, Post-translational Modifications, Ubiquitination
  • Brandname
    None
  • Antibodies applications
    WB, ICC/IF, ELISA
  • Antibody s reactivity
    Human, Mouse, Rat, Bovine
  • Antibody s dilutions
    WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
  • Purity
    Protein G Purified
  • Antibody buffer for storage
    PBS pH7.4, 50% glycerol, 0.09% sodium azide
  • Antibody s concentration
    1 mg/ml
  • Antibody s specificity
    Detects ~10kDa.
  • Storage recommendations
    -20ºC
  • Shipping recommendations
    Blue Ice or 4ºC
  • Antibody certificate of analysis
    1 µg/ml of SMC-160 was sufficient for detection of ubiquitin in 10 µg of Heal Lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
  • Antibody in cell
    Cytoplasm , Nucleus
  • Tissue specificity
    See included datasheet or contact our support service
  • Scientific context
    Ubiquitin is a small protein that occurs in all eukaryotic cells. The ubiquitin protein itself consists of 76 amino acids and has a molecular mass of about 8.5kDa. Key features include its C-terminal tail and the 7 Lys residues. It is highly conserved among eukaryotic species: Human and yeast ubiquitin share 96% sequence identity (1). The main function of Ubiquitin is to clear abnormal, foreign and improperly folded proteins by targeting them for degradation by the 26S proteosome (2). Ubiquitination represents an essential cellular process affected by a multi-enzyme cascade involving classes of enzymes known as ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s or Ubcs) and ubiquitin-protein ligases (E3s). Ubiquitin is activated in a two-step reaction by an E1 ubiquitin-activating enzyme in a process requiring ATP as an energy source. The initial step involves production of an ubiquitin-adenylate intermediate. The second step transfers ubiquitin to the E1 active site cysteine residue, with release of AMP. This step results in a thioester linkage between the C-terminal carboxyl group of ubiquitin and the E1 cysteine sulfhydryl group. The third step is a transfer of ubiquitin from E1 to the active site cysteine of a ubiquitin-conjugating enzyme E2 via a trans(thio)esterification reaction. And the final step of the ubiquitylation cascade creates an isopeptide bond between a lysine of the target protein and the C-terminal glycine of ubiquitin. In general, this step requires the activity of one of the hundreds of E3 ubiquitin-protein ligases (often termed simply ubiquitin ligase). E3 enzymes function as the substrate recognition modules of the system and are capable of interaction with both E2 and substrate(2, 3). Ubiquitination also participates in the internalization and degradation of plasma membrane proteins such as some of the TCR subunits while still ER-membrane associated (4). Ubiquitin also plays a role in regulating signal transduction cascades through the elimination inhibitory proteins, such as IκBα and p27 (5).
  • Bibliography
  • Released date
    1-Apr-2009
  • Tested applications
    To be tested
  • Tested reactivity
    To be tested
  • NCBI number
    NP_776558.1
  • Gene number
    281370
  • Protein number
    P0CG53
  • Antibody s datasheet
    Contact our support service
  • Representative figure link
  • Representative figure legend
    Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Ubiquitin Monoclonal Antibody, Clone 5B9-B3 (SMC-160). Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Mouse Anti-Ubiquitin Monoclonal Antibody (SMC-160) at 1:100 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Diffuse nuclear and cytoplasmic staining. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-Ubiquitin Antibody. (C) Composite. Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Ubiquitin Monoclonal Antibody, Clone 5B9-B3 (SMC-160). Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Mouse Anti-Ubiquitin Monoclonal Antibody (SMC-160) at 1:100 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Diffuse nuclear and cytoplasmic staining. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-Ubiquitin Antibody. (C) Composite. Western Blot analysis of Human cell lysates showing detection of Ubiquitin protein using Mouse Anti-Ubiquitin Monoclonal Antibody, Clone 5B9-B3 (SMC-160). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Ubiquitin Monoclonal Antibody (SMC-160) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT. Mouse Anti-Ubiquitin Antibody [5B9-B3] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human HeLa Cells (SMC-160) Mouse Anti-Ubiquitin Antibody [5B9-B3] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human HeLa Cells (SMC-160) Mouse Anti-Ubiquitin Antibody [5B9-B3] used in Western Blot (WB) on Human cell lysates (SMC-160)
  • Warning information
    Non-hazardous
  • Country of production
    Canada
  • Total weight kg
    1.4
  • Net weight g
    0.2
  • Stock availability
    In Stock
  • Other related products
  • Gene target
    - Ubiquitin Monoclonal IgG2a Kappa 5B9-B3
  • Gene info
    Gene info
    Gene info
    Gene info
  • Gene symbol
    PCDHGB3, IGKV4-1, ITGA3, CYTIP
  • Host
    mouse
  • isotype filter
    • IgG2a Kappa
  • Short name
    Mouse Anti- Ubiquitin Monoclonal IgG2a Kappa, Clone 5B9-B3
  • label filter
    • ATTO 594
  • Clone name
    5B9-B3
  • technique filter
    • anti-
    • Mouse
    • anti
  • Technique
    anti-, Mouse, anti, antibody to, antibodies, Monoclonals or monoclonal antibodies, mouses
  • Alternative technique
    murine, antibodies