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Mouse Anti-7-Ketocholesterol (7-KC) Monoclonal IgG3, Clone 3F7

  • Catalog number
    SMC-513D-HRP
  • Product name
    Mouse Anti-7-Ketocholesterol (7-KC) Monoclonal IgG3, Clone 3F7
  • Size
    100 µg
  • Label
    HRP
  • Clone
    3F7
  • Immunogen
    Synthetic 7-Ketocholesterol conjugated to Keyhole Limpet Hemocyanin (KLH).
  • Antibody s full description
    Mouse Anti- 7-Ketocholesterol Monoclonal IgG3 Antibody, Clone: 3F7: HRP
  • Antibody s category
    Monoclonal Antibodies
  • Antibody s other name
    7-Ketocholesterol [7-KC] Antibody, 7-KC Antibody, 7-ketocholesterol (7-KC) Antibody, 7KC Antibody, 7KC-Modified Keyhole-Lympet Hemocyanine Antibody, 7KC-Modified KLH Antibody
  • Raised in
    Mouse
  • Isotype
    IgG3, IgG3
  • Antibody s target
    7-Ketocholesterol
  • Primary research fields
    Cancer, Oxidative Stress, Lipid peroxidation
  • Brandname
    MOLECULAR SIGNATURE®
  • Antibodies applications
    WB, ICC/IF, ELISA
  • Antibody s reactivity
    Species Independent
  • Antibody s dilutions
    WB (1:1000); ICC/IF (1:50); ELISA (1:1000); optimal dilutions for assays should be determined by the user.
  • Purity
    Protein G Purified
  • Antibody buffer for storage
    PBS pH 7.4, 50% glycerol, 0.9% Sodium Azide
  • Antibody s concentration
    1 mg/ml
  • Antibody s specificity
    Specific for 7-ketocholesterol modified proteins.
  • Storage recommendations
    -20ºC
  • Shipping recommendations
    Blue Ice or 4ºC
  • Antibody certificate of analysis
    A 1:1000 dilution of SMC-513 was sufficient for detection of 7-Ketocholesterol in 2 µg of 7-ketocholesterol conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody.
  • Antibody in cell
    See included datasheet or contact our support service
  • Tissue specificity
    See included datasheet or contact our support service
  • Released date
    23-Mar-2017
  • Tested applications
    To be tested
  • Tested reactivity
    To be tested
  • NCBI number
    Refer to NCBI
  • Gene number
    Refer to GenBank
  • Protein number
    Refer to Swiss-Prot
  • Antibody s datasheet
    Contact our support service
  • Representative figure link
    Representative_figure_link
  • Representative figure legend
    Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-7-Ketocholesterol Monoclonal Antibody, Clone 3F7 (SMC-513). Tissue: Embryonic kidney cells (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-7-Ketocholesterol Monoclonal Antibody (SMC-513) at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250: 1:1000 for 30-60 min at RT. (A,C,E,F) - Untreated. (B,D,F,H) - Cells cultured overnight with 50 µM H2O2. (A,B) DAPI (blue) nuclear stain (C,D) Phalloidin Alex Fluor 633 F-Actin stain (E,F) 7-Ketocholesterol Antibody (G,F) Composite. Courtesy of: Dr. Robert Burke, University of Victoria. Western Blot analysis of 7-Ketocholesterol-BSA Conjugate showing detection of 67 kDa 7-Ketocholesterol-BSA using Mouse Anti-7-Ketocholesterol Monoclonal Antibody, Clone 3F7 (SMC-513). Lane 1: Molecular Weight Ladder (MW). Lane 2: BSA (0.5 µg). Lane 3: BSA (2.0 µg). Lane 4: 7-ketocholesterol-BSA (0.5 µg). Lane 5: 7-ketocholesterol-BSA (2.0 µg). Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-7-Ketocholesterol Monoclonal Antibody (SMC-513) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 67 kDa. Mouse Anti-7-Ketocholesterol Antibody [3F7] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Embryonic kidney cells (HEK293) (SMC-513) Mouse Anti-7-Ketocholesterol Antibody [3F7] used in Western Blot (WB) on 7-Ketocholesterol-BSA Conjugate (SMC-513)
  • Warning information
    Non-hazardous
  • Country of production
    Canada
  • Total weight kg
    1.4
  • Net weight g
    0.1
  • Stock availability
    In Stock
  • Other related products
    Other_related_products
  • Gene target
    7-Ketocholesterol 7-KC Monoclonal IgG3 3F7
    • Gene info
    • Identity:HGNC:37538
    • Gene:PIRC34
    • Long gene name:piwi-interacting RNA cluster 34
    • Discovery year:2009-11-05
    • Entrez gene record:100313890
    • Pubmed identification:17881367
    • Classification:Piwi-interacting RNA clusters
    Gene info
    • Identity:HGNC:37537
    • Gene:PIRC33
    • Long gene name:piwi-interacting RNA cluster 33
    • Discovery year:2009-11-05
    • Entrez gene record:100313854
    • Pubmed identification:17881367
    • Classification:Piwi-interacting RNA clusters
    Gene info
    • Identity:HGNC:37536
    • Gene:PIRC32
    • Long gene name:piwi-interacting RNA cluster 32
    • Discovery year:2009-11-05
    • Entrez gene record:100313811
    • Pubmed identification:17881367
    • Classification:Piwi-interacting RNA clusters
    Gene info
    Gene info
    Gene info
    Gene info
    Gene info
    • Identity:HGNC:37533
    • Gene:PIRC29
    • Long gene name:piwi-interacting RNA cluster 29
    • Discovery year:2009-11-05
    • Entrez gene record:100313927
    • Pubmed identification:17881367
    • Classification:Piwi-interacting RNA clusters
    Gene info
    • Identity:HGNC:37534
    • Gene:PIRC30
    • Long gene name:piwi-interacting RNA cluster 30
    • Discovery year:2009-11-05
    • Entrez gene record:100313853
    • Pubmed identification:17881367
    • Classification:Piwi-interacting RNA clusters
    Gene info
    • Identity:HGNC:37535
    • Gene:PIRC31
    • Long gene name:piwi-interacting RNA cluster 31
    • Discovery year:2009-11-05
    • Entrez gene record:100313787
    • Pubmed identification:17881367
    • Classification:Piwi-interacting RNA clusters
  • Gene symbol
    PIRC34, PIRC33, PIRC32, SNORD114-7, IGHV3OR15-7, SNORD115-7, RNU6-7, PIRC29, PIRC30, PIRC31
  • Host
    mouse
  • isotype filter
    • IgG3
  • Short name
    Mouse Anti-7-Ketocholesterol (7-KC) Monoclonal IgG3, Clone 3F7
  • label filter
    • HRP
  • Clone name
    3F7
  • technique filter
    • anti-
    • Mouse
    • anti
  • Technique
    anti-, Mouse, anti, antibody to, antibodies, Monoclonals or monoclonal antibodies, mouses
  • Alternative technique
    murine, antibodies