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Chicken Anti-Human HSP70 Polyclonal: PE/ATTO 594

  • Catalog number
    SPC-178D-P594
  • Product name
    Chicken Anti-Human HSP70 Polyclonal: PE/ATTO 594
  • Size
    100 µg
  • Label
    PE/ATTO 594
  • Immunogen
    Full length Human HSP70
  • Antibody s target
    Human HSP70
  • Antibody s full description
    Chicken Anti-Human HSP70 Polyclonal
  • Primary research fields
    Cancer, Heat Shock
  • Antibody s category
    Polyclonal Antibodies
  • Antibody s other name
    HSP70 1 Antibody, HSP70 2 Antibody, HSP70.1 Antibody, HSP72 Antibody, HSPA1 Antibody, HSPA1A Antibody, HSPA1B Antibody
  • Verified applications
    WB, ICC/IF, ELISA
  • Raised in
    Chicken
  • Isotype
    IgY
  • Antibody s reactivity
    Human, Mouse, Rat
  • Antibody s recommended dilutions for use
    WB (1:1000), ICC/IF (1:120); optimal dilutions for assays should be determined by the user.
  • Antibody s purified from
    PEG Purified
  • Recommended buffer for storage
    PBS pH7.4, 50% glycerol, 0.09% sodium azide
  • Antibody s concentration
    1 mg/ml
  • Antibody s specificity
    Detects ~70kDa.
  • Storage recommendations
    -20°C
  • Shipping recommendations
    Blue Ice or 4°C
  • Certificate of analysis
    1 µg/ml of SMC-178 was sufficient for detection of HSP70 in 20 µg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-chicken IgG:HRP as the secondary antibody.
  • Antibody in cell
    Cytoplasm
  • Tissue specificity
    See included datasheet or contact our support service.
  • Scientific context
    Scientific_context
  • Bibliography
    1. Balashova N. et al. (2005) J Biol Chem 280: 2186-96. 2. Boorstein W. R., Ziegelhoffer T. & Craig E. A. (1993) J. Mol. Evol.38 (1): 1-17. 3. Rothman J. (1989) Cell 59: 591 -601. 4. DeLuca-Flaherty et al. (1990) Cell 62: 875-887. 5. Bork P., Sander C. & Valencia A. (1992) Proc. Natl Acad. Sci. USA 89: 7290-7294. 6. Fink A.L. (1999) Physiol. Rev. 79: 425-449.
  • Released date
    1/May/2008
  • NCBI number
    NP_005336.3
  • Gene number
    3303
  • Protein number
    P08107
  • PubMed number
    Refer to PubMed
  • Tested applications
    To be tested
  • Tested reactivity
    To be tested
  • Antibody s datasheet
    Contact our support service to receive datasheet or other technical documentation.
  • Representative figure link
    Representative_figure_link
  • Representative figure legend
    Immunocytochemistry/Immunofluorescence analysis using Chicken Anti-Hsp70 Polyclonal Antibody (SPC-178). Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Chicken Anti-Hsp70 Polyclonal Antibody (SPC-178) at 1:120 for 12 hours at 4°C. Secondary Antibody: R-PE Goat Anti-Chicken (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Perinuclear region of cytoplasm. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-Hsp70 Antibody. (C) Composite. Heat Shocked at 42°C for 1h. | Western blot analysis of Human HeLa cell lysates showing detection of HSP70 protein using Chicken Anti-HSP70 Polyclonal Antibody (SPC-178). Load: 15 µg protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Chicken Anti-HSP70 Polyclonal Antibody (SPC-178) at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT. | Immunocytochemistry/Immunofluorescence analysis using Chicken Anti-Hsp70 Polyclonal Antibody (SPC-178). Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Chicken Anti-Hsp70 Polyclonal Antibody (SPC-178) at 1:120 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Chicken (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Perinuclear region of cytoplasm. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-Hsp70 Antibody. (C) Composite. Heat Shocked at 42°C for 1h. Chicken Anti-Hsp70 Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Heat Shocked HeLa Cells (SPC-178) | Chicken Anti-HSP70 Antibody used in Western blot (WB) on Human HeLa cell lysates (SPC-178) | Chicken Anti-Hsp70 Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Heat Shocked HeLa Cells (SPC-178)
  • Warning information
    Non-hazardous
  • Country of production
    Canada
  • Total weight kg
    1.4
  • Net weight g
    0.1
  • Stock availabilit
    In Stock
  • Other related products
    Other_related_products
  • Gene target
    HSP70 PE/ATTO 594
    • Gene info
    Gene info
    Gene info
    Gene info
  • Gene symbol
    HSPA1B, HSPA14, HSPA1A, HSPA1L
  • isotype filter
    • IgY
  • Short name
    Anti- HSP70 Polyclonal: PE/ATTO 594
  • label filter
    • PE ATTO 594
  • technique filter
    • anti-Human
    • Polyclonal
    • anti-
    • anti
  • Technique
    anti-Human, Polyclonal, anti-, anti, antibody to, antibodies, antibodies against human proteins, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
  • Alternative technique
    polyclonals, antibodies