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Human Fc region of immunoglobulin G (IgG Fc) ELISA Kit

  • Catalog number
    EK0206
  • Product name
    Human Fc region of immunoglobulin G (IgG Fc) ELISA Kit
  • Size
    96-wells plate (breakable strips)
  • Short name
    Fc region of immunoglobulin G,Fcγ ELISA
  • Long name
    ELISA test kit for Human Fc region of immunoglobulin G,Fcγ
  • Synonym name
    Homo sapiens Fc region of immunoglobulin G (IgG Fc) ELISA Kit
  • General description
    This is a ready to use kit for performing ELISA tests for detection of Fc region of immunoglobulin G (IgG Fc) in samples from Human
  • Antigen
    Fc region of immunoglobulin G (IgG Fc)
  • Species reactivity
    Human
  • Sample types
    Our Fc region of immunoglobulin G,Fcγ ELISA kit has been proven to work and successfully detect Fc region of immunoglobulin G (IgG Fc) in Human
  • Kit contents
    ELISA test kit for Human Fc region of immunoglobulin G,FcγAssay plate 1 (96wells); Standard 5 x 0.5 ml; A ntibody 1x5ml; Conjugate 1x10ml; Wash buffer 20ml (25X); Substrate A 1x5ml; Substrate B 1x5ml; Stop solution 1x5ml;Adhesive strips (4pcs); working manual
  • Detection wavelengt
    450 nm
  • Assay procedure
    1-4h
  • Summary of the prodecure
    1. Prepare the reagents and samples according to the manual; 2. Leave one well empy; 3. Place 50ul of standard or sample in each well; 4. Add 50ul of the antibody to each well except the Blank (empty); 5 Allow 60 minutes for incubation at 37 degrees celsius; 6. Wash the wells 5 times; 7. Add 100ul of the conjugate to each well but the Blank one; 8. Allow 30 more minutes for incubation at 37C; 9. Wash 5 more times; 10. Add 50ul of both Substrate A and Substrate B in each well (incl. the Blank) and incubate for 15 minutes; 11. Put 50 ul of Stop Solution in all wells (incl. the Blank) and read the results within maximum 10 minutes; Notes: *Samples might require dilution, follow the sample dilution instructions of the manual. **During the last incubation with Substrates A and B the setting should be protected from any light.
  • Applications
    Enzyme Linked Immunosorbent Assay
  • Shipping storage shelf life
    The Fc region of immunoglobulin G,Fcγ ELISA kit is shipped on ice packs. Upon delivery store the kit at +4 C. The shelf life of the Human Fc region of immunoglobulin G (IgG Fc) ELISA Kit is 6 months after delivery.
  • Tips
    Due to transportation and handling small amounts of the liquid components of the Homo sapiens Fc region of immunoglobulin G (IgG Fc) ELISA Kit can get caught on the walls or lid of the vial. Prior to use be sure to briefly centrifuge all vials to ensure that all of the content is on the bottom. Do not freeze the kit, as this might denaturate the standards and the peptide chains of the antibodies used for the production if the ELISA kit. Substrate A and Substrate B are light sensitive, therefore you should protect the kits from direct light. When using the vials of Substrates A and B you may cover them in aluminium foil to protect them from light influences.
  • Datasheet
    Datasheet
  • Alternative to gene target
    • [ "v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog", "C-Kit and CD117 and PBT and SCFR", "KIT and IDBG-18980 and ENSG00000157404 and 3815", "transferase activity", "Extracellular", "Kit and IDBG-172083 and ENSMUSG00000005672 and 16590", "KIT and IDBG-642326 and ENSBTAG00000002699 and 280832" ]
  • Gene target
    Fc region of immunoglobulin G Fc
  • Host
    antigen assay ELISA kit 1 Kit (96 Wells)
  • Isotype
    IgG, IgG
  • isotype filter
    • IgG
  • technique filter
    • ELISA kit
    • ELISA
    • IgG
    • FC
  • Technique
    ELISA kit, ELISA, IgG, FC, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, mouse, … proteins are supplied in coated 96 well plates to be stored at +4°C. ELISA test kits can be sandwich ELISA. In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies. In single antibody ELISAs the antigen is coated and only a detector antibody is used. Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen. In this case the ratio on your graph will be inverse proportional with the antigen present in your sample. A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion. Genprice Inc. the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800. MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay. PROMT code 3841327 Immunoabsorbent assay tests. ELISA tests are enzyme linked immunoassays to detect human, mouse or other proteins in serum, plasma, urine or biological fluids. MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits. Also Enzyme-linked immunoassay, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330. This is the desigation of other diagnostic analyzers and tests. IgGs
  • Alternative technique
    elisas, kits, immunoglobulins
  • Tissue
    region
  • tissue filter
    • region