gentaur logogentaur logo
x

anti-Synapsin (phospho Ser9) Antibody

  • Catalog number
    XPS-1025
  • Product name
    anti-Synapsin (phospho Ser9) Antibody
  • Size
    0.1 mL
  • Gene info
    Synapsin I plays a key role in synaptic plasticity in brain. This effect is due in large part to the ability of the synapsins to regulate the availability of synaptic vesicles for release. In addition to its role in plasticity, the expression of synapsin I is a precise indicator of synapse formation. Thus synapsin I immunocytochemistry provides a valuable tool for the study of synaptogenesis. The role of synapsin in synaptic plasticity and in synaptogensis is regulated by phosphorylation. Serine 9 is the site on synapsin I that is phosphorylated by cAMP-dependent protein kinase and by calcium calmodulin kinase I. Phosphorylation of this site is thought to regulate synaptic vesicle function.
  • Gene names
    Synapsin I,
  • Antibodie s label
    unlabelled serum
  • Purity
    Affinity Purified
  • Ab raised in
    Rabbit
  • Type
    Polyclonal
  • Spicific to
    Synapsin antibody is specific for Synapsin I phosphorylated at Ser9.
  • Antigen
    Synapsin (Ser9) polyclonal antibody was made against a synthetic phosphopeptide corresponding to amino acids residues surrounding the phosphorylated Ser9 of Rat Synapsin I.
  • Immuno application
    Western Blot, immuno-histo-chemistry
  • Antibody advice
    Immunolabeling of the 78k Synapsin I doublet in a Western blot of rat brain homogenate is blocked by the phosphopeptide used as antigen but not by the corresponding non-phosphopeptide. The antibody also weakly labels Synapsin II (55k) which has a similar phosphorylation site to that of Ser9 on Synapsin I. Applications include Dot Blots (DB) and Western Blots (WB). Suitability for Immunohistochemistry (IHC) has not yet been determined. Human, mouse, rat and Xenopus have 100% amino acid sequence identity with the antigen used to raise the antibody. When internally tested under ideal conditions the working dilutions were 1:1000 for DB and WB.
  • Suspention pH azide
    100 uL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per mL BSA and 50% glycerol.
  • Keep at
    For long term storage –80 grades C is recommended, but shorter term storage at –20 grades C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stable for one year.
  • Works with
    Human, Mouse, Rat, Xenopus
  • Omim nr
    1351166
  • Ncbi nr
    P17599
  • KDa
    78
  • Vial with antibody
    in solution
  • NCBI GENE nr
    281510
  • GENE symbol
    SYN1
  • Antigene name
    synapsin I
  • Source
    Bos taurus
  • Protein nr
    P17599
  • Picture
    Picture
  • Pdf tech file
    Pdf_tech_file
  • More antibodies
    More_antibodies
  • Gene target
    Synapsin phospho Ser9
  • Short name
    anti-Synapsin (phospho Ser9) Antibody
  • technique filter
    • Antibody
    • anti-
    • anti
  • Technique
    Antibody, anti-, anti, antibody to, antibodies, antibodies against human proteins, antibodies for
  • Alternative technique
    antibodies