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Renilla Luciferase Assay System

  • Catalog number
    PKSH031680-200µg
  • Product name
    Recombinant Human GFRA1 / GFRα1 / GDNFRA Protein (His tag) 200µg
  • Size
    200µg
  • Synonym
    GDNFR;GDNFRA;GFR-ALPHA-1;RET1L;RETL1;TRNR1
  • Activity
    Measured in a cell proliferation assay using SH‑SY5Y human neuroblastoma cells. The ED50 for this effect is typically 0.2-1 μg/mL in the presence of 40 ng/mL Recombinant Human GDNF.2. Measured by its ability to bind human GDNF (native) in functional ELISA.
  • Sequence
    Met 1-Ser 424
  • Fusion tag
    C-His
  • Accession
    NP_665736.1
  • Expressed Host
    HEK293 Cells
  • Shipping
    In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
  • Purity
    ＞98 % as determined by SDS-PAGE
  • Endotoxin
    ＜1.0 EU per µg as determined by the LAL method
  • Stability and Storage
    Samples are stable for up to twelve months from date of receipt at -70℃.Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Mol Mass
    46 kDa
  • AP Mol Mass
    55-60 kDa
  • Formulation
    Lyophilized from sterile PBS, pH 7.4
  • Reconstitution
    Please refer to it for detailed information.
  • Background
    Glial cell line derived neurotrophic factor (GDNF) Family Receptor Alpha 1 (GFRA1) is a member of the GDNF receptor family. It is a glycosylphosphatidylinositol (GPI)-linked cell surface receptor for both GDNF and NTN, and mediates activation of the RET tyrosine kinase receptor. GFRA1 is a potent survival factor for central and peripheral neurons, and is essential for the development of kidneys and the enteric nervous system. Glial cell line-derived neurotrophic factor (GDNF) and neurturin (NTN) are its binding ligand which are two structurally related, potent neurotrophic factors that play key roles in the control of neuron survival and differentiation. GDNF promotes the formation of a physical complex between GFRA/GDNFRa and the orphan tyrosin kinase receptor Ret, thereby inducing its tyrosine phosphorylation. The RET is a receptor tyrosine kinase representing the signal-transducing molecule of a multisubunit surface receptor complex for the GDNF, in which GFRA / GDNFRa acts as the ligand-binding component. GDNF, a distantly related member of the transforming growth factor-β (TGF-â) superfamily, and its receptor components: GFRA1, Ret and neural cell adhesion molecule (NCAM) have been recently reported to be expressed in the testis and to be involved in the proliferation regulation of immature Sertoli cells.
  • Product URL
    Product_URL
  • PDF URL
    PDF_URL
  • Gene target
    GFRA1 / GFRα1 / GDNFRA Protein His tag 200µg
    • Gene info
    Gene info
  • Gene symbol
    GFRA1, RAPGEF5
  • Short name
    Recombinant GFRA1 / GFRα1 / GDNFRA Protein (His tag) 200µg
  • Label
    His
  • label filter
    • His
  • technique filter
    • Recombinant
  • Technique
    Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Elabscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture. Tags
  • Alternative technique
    rec