Recombinant Human Noggin/NOG (N-8His-Flag)
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Catalog number
C018-50
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Price
Please ask
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Size
50 ug
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Description
Recombinant Human Noggin is produced by our Mammalian expression system and the target gene encoding Gln28-Cys232 is expressed with a 8His-Flag tag at the N-terminus.
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Species reactivity
Human
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Origin
Human cells
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Peptide sequence
HHHHHHHHDYKDDDDKQHYLHIRPAPSDNLPLVDLIEHPDPIFDPKEKDLNETLLRSLLGGHYDPGFMATSPPEDRPGGGGGAAGGAEDLAELDQLLRQRPSGAMPSEIKGLEFSEGLAQGKKQRLSKKLRRKLQMWLWSQTFCPVLYAWNDLGSRFWPRYVKVGSCFSKRSCSVPEGMVCKPSKSVHLTVLRWRCQRRGGQRCGWIPIQYPIISECKCSC
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Estimated molecular weight
25,2 kDa
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Protein purity
Greater than 95% as determined by reducing SDS-PAGE.
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Endotoxin level
Less than 0.1 ng/µg (1 IEU/µg) as determined by LAL test.
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Shipping condition
Ambient/Room Temperature
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Package form
Lyophilized from a 0.2 µm filtered solution of 20mM PB,500mM NaCl,2mM EDTA,pH7.4.
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Storage conditions
Lyophilized protein should be stored at below -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at below -20°C for 3 months.
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Reconstitution conditions
See included datasheet or contact us for more information.
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UniProt number
Q13253
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Properties
An anti-flag tag (FLAG fusion protein) is use to detect a FLAG-tag, or FLAG octapeptide, or FLAG epitope that is a polypeptide protein tag that can be added to a protein using recombinant DNA. This FLAG-tags have the sequence DYKDDDDK motiv. These tags are very useful to do protein purification by affinity chromatography. Also separation of recombinant, overexpressed proteins from cell lysates is done by FLAG go HIS tags. FLAGS are also used in the isolation of protein complexes with multiple subunits, because its mild purification procedure tends not to disrupt such complexes. It has been used to enrich proteins of height purity and quality to see the 3D crystal structure with x-ray. Suitable for in vivo use in cells. For electrophorese protein detection rabbit polyclonals anti Flag conjugation are the most suited antibodies. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Conjugation
Flag
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Source
Recombinants or rec. proteins
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Group
recombinants
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Gene target
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Gene symbol
NOG
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Short name
Recombinant Noggin/NOG (N-8His- )
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Technique
Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. novo advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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Label
Flag
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Species
Human, Humans
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Alternative name
Recombinant Human Noggin (N-8His-Flag)
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Alternative technique
rec
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Alternative to gene target
noggin, SYM1 and SYNS1, NOG and IDBG-60160 and ENSG00000183691 and 9241, protein homodimerization activity, Extracellular, Nog and IDBG-208239 and ENSMUSG00000048616 and 18121, NOG and IDBG-647068 and ENSBTAG00000040282 and 538769
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Gene info
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Identity
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Gene
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Long gene name
noggin
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Synonyms gene
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Synonyms gene name
- synostoses (multiple) syndrome 1
- symphalangism 1 (proximal)
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GenBank acession
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Locus
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Discovery year
1999-03-24
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
MeSH Data
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Name
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Concept
Scope note:
The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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