- Catalog numberX1211
- Product nameRal B Recombinant Protein
- Size25 μg
- PriceAsk For Price
- CategoryProteins & Peptides
- Long descriptionRalB is a member of the Ras-like GTP binding protein family. Ras proteins provide a crucial link in the intracellular signalling cascades. Ras proteins are members of the small G protein family. These proteins are characterized by a molecular weight between 20 and 30 kDa, the ability to bind GTP or GDP and a low intrinsic GTPase activity. These proteins relay signals from the plasma membrane activated by receptor tyrosine kinases to the nucleus via a network of serine/threonine kinases. Ras proteins belong to a family of approximately 40 mammalian and 60 non-mammalian proteins. Ras proteins were first discovered as the hyperactive products of mutant Ras genes which promote cancer by disrupting the normal controls on cell proliferation and differentiation; about 30% of human cancers have mutations in a Ras gene. Ras and Ras-like proteins function as switches, cycling between 2 distinct conformational states, active when GTP is bound and inactive when GDP is bound. Affinity precipitation assays can be used to determine the activation status of Ras and Ras-like family members. This assay is based on the ability of the Ras family effector proteins to interact specifically with the activated GTP-bound form of their target GTPases.
- Antibody come fromRecombinant GST protein from E. Coli
- Other descriptionProvided in 50mM Tris (pH 7.5) solution containing 150 mM NaCl, 10 mM MgCl2, 1 mM DTT, 10% glycerol.
- Clonenot specified
- Isotypenot specified
- Antigen antibody binding interactionRal B Recombinant Protein
- Antibody is raised insee techfile
- Antibody s reacts withHuman
- Antibody s reacts with these speciesThis antibody doesn't cross react with other species
- Labelunlabeled
- Antibody s specificityNo Data Available
- ApplicationAffinity Binding
- Antibody s suited forRal B protein is provided as a functional G-protein and can be used as a Western blot control for antibodies to Ral B protein or in assays to study to protein-protein interactions of Ral B and related proteins with regulatory proteins.
- Storage-80ºC
- Relevant references1. Zhao, Z. & Rivkees, S.A., Tissue-specific expression of GTPase RalA and RalB during embryogenesis and regulation by epithelial-mesenchymal interaction. Mech. Dev. 2000, 97, 201-204 _x000B__x000B_2. Bhullar, R.P. & Yang, S., Immunodetection of ralA and ralB GTP-binding proteins in various rat tissues and platelets. Mol. Cell Biochem. 1998, 179, 49-55_x000B__x000B_ 3. Jilkina, O. & Bhullar, R.P., Generation of antibodies specific for the RalA and RalB GTP-binding proteins and determination of their concentration and distribution in human platelets. Biochim. Biophys. Acta 1996, 1314, 157-166
- Protein numbersee ncbi
- WarningsThis product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
- Antibody s link
- Gene targetRal B Protein
- Identity:HGNC:9840
- Gene:RALB
- Long gene name:RAS like proto-oncogene B
- Synonyms gene name:v-ral simian leukemia viral oncogene homolog B
- Discovery year:1989-06-30
- Entrez gene record:5899
- RefSeq identity:NM_002881
- Classification:RAS type GTPase family
- VEGA ID:OTTHUMG00000131435
- Gene symbolRALB
- isotype filter
- not specified
- Short nameRal B Recombinant Protein
- label filter
- unlabeled
- technique filter
- Recombinant
- TechniqueRecombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. nordc advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
- Alternative techniquerec