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Rabbit anti Mouse IgM (Fc specific) antibody conjugated with Horseradish peroxidase

  • Catalog number
    RAM/IgM(Fc)/PO
  • Product name
    Rabbit anti Mouse IgM (Fc specific) antibody conjugated with Horseradish peroxidase
  • Size
    1 mL
  • Category
    Secondary Antibodies
  • Long description
    The reactivity of the antiserum is directed to the Fc subunit of the IgM molecule which expresses strict isotypic (class) specificity. It does not react with purified IgG, IgG/Fab fragments and IgA or any non-Ig protein in mouse serum, as tested by immunoelectrophoresis and double radial immunodiffusion. In enzyme-immunocytochemical and immunohistochemical staining for the detection of IgM at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of mouse origin known to be of the IgM isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgM in mouse serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions for histochemical and cytochemical use are usually between 1:100 and 1:500; in ELISA and comparable non-precipitating antibody-binding assays between 1:1.000 and 1:10,000 depending on the method used.
  • Antibody come from
    Purified homogenous IgM isolated from pooled mouse serum. Immunization with intact (19S) and split IgM (7S). Freund’s complete adjuvant is used in the first step of the immunization procedure
  • Other description
    Peroxidase-coupled purified hyperimmune rabbit IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity
  • Clone
    Polyclonal
  • Isotype
    not specified, IgM
  • Antigen antibody binding interaction
    Rabbit anti Mouse IgM (Fc specific) antibody conjugated with Horseradish peroxidase Antibody
  • Antibody is raised in
    Rabbit
  • Antibody s reacts with
    Mouse
  • Antibody s reacts with these species
    Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail, however in double radial diffusion a reaction with Rat IgM has been observed.
  • Label
    HRP
  • Antibody s specificity
    Horseradish peroxidase-conjugated IgG fraction of polyclonal Rabbit antiSerum to Mouse IgM, Fc specific
  • Research interest
    Veterinary
  • Application
    ELISA,Immunocytochemistry,Immunohistochemistry (paraffin),Dot blot,Immunoblotting
  • Antibody s suited for
    ELISA,Immunocytochemistry,Immunohistochemistry (paraffin),Dot blot,Immunoblotting.
  • Storage
    The lyophilized conjugate is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. It is reconstituted by adding 1 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate. Lyophilized at +4°C--at least 10 years. Reconstituted at or below -20°C--3-5 years. Reconstituted at +4°C--7 days
  • Relevant references
    no information yet
  • Protein number
    see ncbi
  • Warnings
    This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This product contains sodium azide. To prevent formation of toxic vapors, do not mix with strong acidic solutions. To prevent formation of potentially explosive metallic azides in metal plumbing, always wash into drain with copious quantities of water. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
  • Antibody s link
    Antibody_s_link
  • Gene target
    Fc specific conjugated with Horseradish
  • Host
    Rabbit, Rabbits
  • isotype filter
    • not specified
  • Short name
    Rabbit anti Mouse IgM (Fc specific) antibody conjugated with Horseradish
  • label filter
    • HRP
  • technique filter
    • anti mouse
    • Antibody
    • Rabbit
    • Mouse
    • anti
    • IgM
    • FC
  • Technique
    anti mouse, Antibody, Rabbit, Mouse, anti, IgM, FC, antibody to, anti mouse antibodies, antibodies against human proteins, antibodies for, antibody Conjugates, IgMs, mouses
  • Alternative technique
    antibodies, rabbit-anti, murine, immunoglobulin-m