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Rabbit anti Mouse IgG1 IgG2a IgG2b IgG3 IgA IgM (heavy and light chains) antibody conjugated with Biotin

  • Catalog number
    RAM/Ig/Bio
  • Product name
    Rabbit anti Mouse IgG1 IgG2a IgG2b IgG3 IgA IgM (heavy and light chains) antibody conjugated with Biotin
  • Size
    1 mL
  • Category
    Secondary Antibodies
  • Long description
    The reactivity of the antiserum is directed the major immunoglobulin isotypes of the mouse Ig system (classes, subclasses and L-chain types), including antibodies to common determinants, to class and subclass-specific determinants, and to the surface determinants of the common Fab portion, as tested by immunoelectrophoresis and double radial immunodiffusion using various antiserum concentrations against normal mouse plasma and serum. In immunocytochemical and immunohistochemical staining of immunoglobulins at the cellular and subcellular level of appropriately treated cell and tissue substrates; to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of mouse origin in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure immunoglobulins in mouse serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions for histochemical and cytochemical use are usually between 1:100 and 1:500; in ELISA and comparable non-precipitating antibody-binding assays between 1:2,000 and 1:20,000.
  • Antibody come from
    Purified polyclonal IgG and pools of homogenous IgA and IgM isolated from mouse serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
  • Other description
    Biotin-coupled purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity.
  • Clone
    Polyclonal
  • Isotype
    not specified, IgA, IgG1, IgG2a, IgG2b, IgG3, IgM
  • Antigen antibody binding interaction
    Rabbit anti Mouse IgG1 IgG2a IgG2b IgG3 IgA IgM (heavy and light chains) antibody conjugated with Biotin Antibody
  • Antibody is raised in
    Rabbit
  • Antibody s reacts with
    Mouse
  • Antibody s reacts with these species
    Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. The antiSerum has been tested for cross-reactivity in double radial immunodiffusion with the following results: Bovine + Duck - Horse + Rat ++ Cat + Goat + Human + Sheep + Chicken - Guinea Pig ++ Monkey + Swine + Dog + Hamster ++ pigeon - Turkey ± A negative cross-reaction in double radial immunodiffusion does not exclude some reaction in more sensitive techniques.
  • Label
    Biotin
  • Antibody s specificity
    Biotin-conjugated IgG fraction of polyclonal Goat antiSerum to Mouse IgG, IgA and IgM, heavy and light chains
  • Research interest
    Veterinary
  • Application
    ELISA,Immunocytochemistry,Immunohistochemistry (paraffin),Dot blot,Immunoblotting
  • Antibody s suited for
    ELISA,Immunocytochemistry,Immunohistochemistry (paraffin),Dot blot,Immunoblotting.
  • Storage
    The lyophilized conjugate is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. It is reconstituted by adding 1 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate. Lyophilized at +4°C--at least 10 years. Reconstituted at or below -20°C--3-5 years. Reconstituted at +4°C--7 days
  • Relevant references
    no information yet
  • Protein number
    see ncbi
  • Warnings
    This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This product contains sodium azide. To prevent formation of toxic vapors, do not mix with strong acidic solutions. To prevent formation of potentially explosive metallic azides in metal plumbing, always wash into drain with copious quantities of water. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
  • Antibody s link
    Antibody_s_link
  • Gene target
    IgG1 IgG2a IgG2b IgG3 heavy light chains conjugated with
  • Host
    Rabbit, Rabbits
  • isotype filter
    • not specified
  • Short name
    Rabbit anti Mouse IgG1 IgG2a IgG2b IgG3 IgA IgM (heavy light chains) antibody conjugated with
  • label filter
    • Biotin
  • technique filter
    • anti mouse
    • Antibody
    • Rabbit
    • light
    • Mouse
    • anti
    • IgA
    • IgM
  • Technique
    anti mouse, Antibody, Rabbit, light, Mouse, anti, IgA, IgM, antibody to, anti mouse antibodies, antibodies against human proteins, antibodies for, antibody Conjugates, IgAs, IgMs, mouses
  • Alternative technique
    antibodies, rabbit-anti, murine, immunoglobulin-a, immunoglobulin-m