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Rabbit anti Mouse IgA (Fc specific) antibody conjugated with TRITC

  • Catalog number
    RAM/IgA(Fc)/TRITC
  • Product name
    Rabbit anti Mouse IgA (Fc specific) antibody conjugated with TRITC
  • Size
    1 mL
  • Category
    Secondary Antibodies
  • Long description
    The reactivity of the antiserum is directed to the Fc subunit of the IgA molecule which expresses strict isotypic (class) specificity. It does not react with any non-Ig protein in mouse serum, as tested by immunoelectrophoresis and double radial immunodiffusion. Direct immunofluorescence staining of cytoplasmic Ig of appropriately treated cell and tissue substrates; to demonstrate immunoglobulins or specific antibodies in cells and tissues; to identify circulating antibodies in serodiagnostic microbiology and autoimmune diseases. Antisera to IgA do not discriminate between serum IgA (monomeric and dimeric) and higher molecular forms such as secretory IgA. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions are usually between 1:10 and 1:40.
  • Antibody come from
    Purified pools of homogenous IgA isolated from pooled mouse serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
  • Other description
    TRITC-coupled purified hyperimmune rabbit IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity.
  • Clone
    Polyclonal
  • Isotype
    not specified, IgA
  • Antigen antibody binding interaction
    Rabbit anti Mouse IgA (Fc specific) antibody conjugated with TRITC Antibody
  • Antibody is raised in
    Rabbit
  • Antibody s reacts with
    Mouse
  • Antibody s reacts with these species
    Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this immunoconjugate has not been tested in detail.
  • Label
    TRITC
  • Antibody s specificity
    Tetramethylrhodamine isothiocyanate-conjugated IgG fraction of polyclonal Rabbit antiSerum to Mouse IgA, Fc specific
  • Research interest
    Veterinary
  • Application
    ELISA,Immunocytochemistry,Immunohistochemistry (frozen),(In)direct immunofluorescence
  • Antibody s suited for
    ELISA,Immunocytochemistry,Immunohistochemistry (frozen),(In)direct immunofluorescence.
  • Storage
    The lyophilized conjugate is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. It is reconstituted by adding 1 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate. Lyophilized at +4°C--at least 10 years. Reconstituted at or below -20°C--3-5 years. Reconstituted at +4°C--7 days
  • Relevant references
    no information yet
  • Protein number
    see ncbi
  • Warnings
    This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This product contains sodium azide. To prevent formation of toxic vapors, do not mix with strong acidic solutions. To prevent formation of potentially explosive metallic azides in metal plumbing, always wash into drain with copious quantities of water. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
  • Antibody s link
    Antibody_s_link
  • Gene target
    Fc specific conjugated with TRITC
  • Host
    Rabbit, Rabbits
  • isotype filter
    • not specified
  • Short name
    Rabbit anti Mouse IgA (Fc specific) antibody conjugated with TRITC
  • label filter
    • TRITC
  • technique filter
    • anti mouse
    • Antibody
    • Rabbit
    • Mouse
    • anti
    • IgA
    • FC
  • Technique
    anti mouse, Antibody, Rabbit, Mouse, anti, IgA, FC, antibody to, anti mouse antibodies, antibodies against human proteins, antibodies for, antibody Conjugates, IgAs, mouses
  • Alternative technique
    antibodies, rabbit-anti, murine, immunoglobulin-a