gentaur logogentaur logo
x

Rabbit anti Horse albumin

  • Catalog number
    RAHo/Alb
  • Product name
    Rabbit anti Horse albumin
  • Size
    1 mL
  • Category
    Primary Antibodies
  • Long description
    The defined antibody specificity is directed to albumin as tested against horse sera. In immunoelectrophoresis and double radial immunodiffusion (Ouchterlony), using various antiserum concentrations against appropriate concentrations of the immunogen, a single characteristic precipitin line is obtained which shows a reaction of identity with the precipitin lines obtained against horse serum and the purified albumin. As precipitating antiserum to identify or measure horse albumin by a variety of immunodiffusion techniques, including immunoelectrophoresis, single and double radial immunodiffusion (Mancini, Ouchterlony) and electroimmunodiffusion (Laurell). It has not been tested for use in nephelometry, ELISA or immunochemistry, but this does not exclude such use if proper controls are included.
  • Antibody come from
    Highly purified albumin isolated from horse serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
  • Other description
    Delipidated, heat inactivated, lyophilized, stable whole antiserum. No preservative added. Total protein and IgG concentrations in the antiserum are comparable to those in pooled rabbit serum. No foreign proteins added.
  • Clone
    Polyclonal
  • Isotype
    not specified
  • Antigen antibody binding interaction
    Rabbit anti Horse albumin Antibody
  • Antibody is raised in
    Rabbit
  • Antibody s reacts with
    Equine
  • Antibody s reacts with these species
    Inter-species cross-reactivity is a normal feature of antibodies to Serum proteins, since homologous proteins of different species frequently share antigenic determinants. The degree of cross-reactivity is also dependent on the concentRations of the reactants and the sensitivity of the assay arrangement. This antiSerum has been tested for cross-reactivity in double radial immunodiffusion with the following results: Bovine + Cat + Chicken - Dog + Duck - Goat + Guinea Pig + Hamster ++ Human + Monkey ++ Mouse + pigeon - Rabbit ± Rat + Sheep + Swine + A negative cross-reaction in double radial immunodiffusion does not exclude some reaction in more sensitive techniques.
  • Label
    unlabeled
  • Antibody s specificity
    Precipitating polyclonal Rabbit antiSerum to Horse Serum albumin.
  • Research interest
    Veterinary
  • Application
    Immunoprecipitation
  • Antibody s suited for
    Immunoprecipitation. In immunoelectrophoresis use 2 μl serum, plasma or equivalent against 120 μl antiserum. In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 μl antiserum in a 3 mm diameter centre well and 2 μl serum samples (neat and serially diluted) in 2 mm diameter peripheral wells. In single radial immunodiffusion and electroimmunodiffusion use 0.5 to 1.0 percent antiserum in the agar gel. Precipitin titre not less than 1:64 when tested against normal horse serum in agar block immunodiffusion titration.
  • Storage
    The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Reconstitute the lyophilized antiserum by adding 1 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4°C, not refrozen, and preferably used the same day. Lyophilized at +4°C--at least 10 years. Reconstituted at or below -20°C--3-5 years. Reconstituted at +4°C--7 days.
  • Relevant references
    no information yet
  • Protein number
    see ncbi
  • Warnings
    This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This product contains sodium azide. To prevent formation of toxic vapors, do not mix with strong acidic solutions. To prevent formation of potentially explosive metallic azides in metal plumbing, always wash into drain with copious quantities of water. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
  • Antibody s link
    Antibody_s_link
  • Gene target
    albumin
    • Gene info
    Locus Specific Databases:Albumin mutation database
    Gene info
    Gene info
    Locus Specific Databases:Global Variome shared LOVDLRG_522
  • Gene symbol
    ALB, DBP, HNF1A
  • Host
    Rabbit, Rabbits
  • isotype filter
    • not specified
  • Short name
    Rabbit anti albumin
  • label filter
    • unlabeled
  • technique filter
    • Rabbit
    • anti
  • Technique
    Rabbit, anti, antibody to
  • Alternative technique
    rabbit-anti, antibodies