- Catalog numberRAGp/IgG2
- Product nameRabbit anti Guinea Pig IgG2 (subclass specific)
- Size1 mL
- PriceAsk For Price
- CategorySecondary Antibodies
- Long descriptionThe reactivity of the antiserum is directed to the Fc subunit of subclass IgG2. It does not react with other IgG1, IgG/Fab fragments, IgM and IgA or any non-Ig protein in guinea pig serum, as tested by immunoelectrophoresis and double radial immunodiffusion. In precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence of IgG2 in guinea pig serum and other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of guinea pig IgG2 from serum or plasma.
- Antibody come fromPools of purified normal IgG2 isolated from pooled guinea pig serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
- Other descriptionDelipidated, heat inactivated, lyophilized whole antiserum. No preservative added, as it may interfere with the antibody activity Total protein and IgG concentration in the antiserum are comparable to those of pooled normal rabbit serum. No foreign proteins added.
- ClonePolyclonal
- Isotypenot specified
- Antigen antibody binding interactionRabbit anti Guinea Pig IgG2 (subclass specific) Antibody
- Antibody is raised inRabbit
- Antibody s reacts withGuinea Pig
- Antibody s reacts with these speciesThis IgG fraction is not species-specific since inter-species cross-reactivity is a normal feature of antisera to immunoglobulins. Cross-reactivity of this product has not been tested in detail.
- Labelunlabeled
- Antibody s specificityPrecipitating polyclonal Rabbit antiSerum to Guinea Pig IgG2, subclass specific.
- Research interestVeterinary
- ApplicationImmunoprecipitation
- Antibody s suited forImmunoprecipitation. In immunoelectrophoresis use 2 μl or equivalent against 120 μl antiserum. In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 μl antiserum in a 3 mm diameter centre well and 2 μl serum samples (neat and diluted) in 2 mm diameter peripheral wells. Precipitin titre not less then 1:32 when tested against pooled normal guinea pig serum in agar block immunodiffusion titration.
- StorageThe lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Reconstitute the lyophilized antiserum by adding 1 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4°C, not refrozen, and preferably used the same day Lyophilized at +4°C--at least 10 years. Reconstituted at or below -20°C--3-5 years. Reconstituted at +4°C--7 days.
- Relevant referencesno information yet
- Protein numbersee ncbi
- WarningsThis product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This product contains sodium azide. To prevent formation of toxic vapors, do not mix with strong acidic solutions. To prevent formation of potentially explosive metallic azides in metal plumbing, always wash into drain with copious quantities of water. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
- Antibody s link
- Gene targetIgG2 subclass specific
- HostRabbit, Rabbits
- isotype filter
- not specified
- Short nameRabbit anti IgG2 (subclass specific)
- label filter
- unlabeled
- technique filter
- Rabbit
- anti
- TechniqueRabbit, anti, antibody to
- Alternative techniquerabbit-anti, antibodies