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Rabbit anti Ceramide Kinase

  • Catalog number
    X1715P
  • Product name
    Rabbit anti Ceramide Kinase
  • Size
    100 μg
  • Category
    Primary Antibodies
  • Long description
    Sphingolipids, in addition to being structural components of membranes, regulate cell-cell and cell-substrate interactions, proliferation, and differentiation. Members of this diverse group of lipids have emerged as a novel class of signaling molecules that also regulate phagocytosis. The mechanisms by which sphingolipids exert these effects remain incompletely defined. More than a decade ago, it was found that ceramide can be phosphorylated to ceramide 1-phosphate (C1P). Ceramide kinase (CERK) and its phosphorylated product ceramide 1-phosphate (C1P) are central players in inflammation and cancer. The product of CERK activity, ceramide 1-phosphate (C1P), has been reported to have mitogenic effects. C1P is a direct activator of cytosolic phospholipase A2 and is involved in arachidonic acid release. CERK is a mediator of Ca2+-dependent degranulation in mast cells. In both arachidonic acid release and mast cell degranulation, the intracellular elevation of Ca2+ is a central event that acts as a regulatory mechanism of CERK activity. C1P is found in brain synaptic vesicles, and plays a role in regulating the secretion of neurotransmitters. CERK activity exists in HL-60 cells where the C1P is derived from ceramide released from sphingomyelin. The expressed kinase has specific ceramide phosphorylating activity. CERKs exist in a variety of cellular organisms, including plants, nematodes, insects, and vertebrates.
  • Antibody come from
    Hybridoma produced by the fusion of splenocytes from (BALB/c x B6)F1 mice immunized with cytoskeletal preparation extracted from human epidermis and mouse SP2/0 myeloma cells.
  • Other description
    Provided as solution in phosphate buffered saline with 0.08% sodium azide. Ammonium Sulfate Precipitation
  • Clone
    Polyclonal
  • Isotype
    not specified
  • Antigen antibody binding interaction
    Rabbit anti Ceramide Kinase Antibody
  • Antibody is raised in
    Rabbit
  • Antibody s reacts with
    Human
  • Antibody s reacts with these species
    This antibody doesn't cross react with other species
  • Label
    unlabeled
  • Antibody s specificity
    No Data Available
  • Application
    Western Blot, ELISA
  • Antibody s suited for
    Antibody can be used for immunohistochemistry on frozen and paraffin-emdedded tissues, immunocytochemistry, immunoblotting (1:100-1:1000) and flow cytomeetry (1:100-1:200). Optimal concentration should be evaluated by serial dilutions.
  • Storage
    -20ºC
  • Relevant references
    1. Ivanyi, D., et al. (1989). New monoclonal antibodies recognizing epidermal differentiation- associated keratins in formalin-fixed, paraffin-embedded tissue. Keratin 10 expression in carcinoma of the vulva, J Pathol 159, 7-12._x000B_2. Ivanyi, D., et al. (1989). Absence of differentiationrelated expression of keratin 10 in early stages of vulvar squamous carcinoma, Differentiation 42, 124-9._x000B_3. Ivanyi, D., et al. (1990). Keratin subtypes in carcinomas of the uterine cervix: implications for histogenesis and differential diagnosis, Cancer Res 50, 5143-52._x000B_4. Ivanyi, D., et al. (1992). Patterns of expression of feline cytokeratins in healthy epithelia and mammary carcinoma cells, Am J Vet Res 53, 304-14._x000B_5. Vos, J. H., et al. (1992). Immunohistochemistry with keratin monoclonal antibodies in canine tissues: urogenital tract, respiratory tract, (neuro-)endocrine tissues, choroid plexus and spinal cord, J Vet Med 39, 721-40._x000B_6. Ivanyi, D., et al. (1993). Cytokeratins as markers of initial stages of squamous metaplasia in feline mammary carcinomas, Am J Vet Res 54, 1095-102._x000B_7. Vos, J. H., et al. (1993). The expression of keratins, vimentin, neurofilament proteins, smooth muscle actin, neuron-specific enolase, and synaptophysin in tumors of the specific glands in the canine anal region, Vet Pathol 30, 352-61_x000B_8. van Bommel, P. F., et al. (1994). Expression of cytokeratin 10, 13, and involucrin as prognostic factors in low stage squamous cell carcinoma of the uterine cervix, Cancer 74, 2314-20.
  • Protein number
    see ncbi
  • Warnings
    This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
  • Antibody s link
    Antibody_s_link
  • Gene target
    Ceramide Kinase
    • Gene info
    Gene info
  • Gene symbol
    CERKL, CERK
  • Host
    Rabbit, Rabbits
  • isotype filter
    • not specified
  • Short name
    Rabbit anti Ceramide Kinase
  • label filter
    • unlabeled
  • technique filter
    • Rabbit
    • anti
  • Technique
    Rabbit, anti, antibody to
  • Alternative technique
    rabbit-anti, antibodies