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Mouse anti p34 cdc-2 nuclear protein

  • Catalog number
    X1236M
  • Product name
    Mouse anti p34 cdc-2 nuclear protein
  • Size
    100 μg
  • Category
    Primary Antibodies
  • Long description
    p34(Cdc2) kinase is a an essential nuclear protein for mitosis in human cells. During the prophase/metaphase transition the inactive phosphorylated form of p34(cdc2) is dephosphorylated to make the active form of the protein. This dephosphorylation is performed by the p80(cdc25) dual-specificity phosphatase. The regulatory subunit of p34(cdc2) is cyclin B, a 47-52 kDa protein. The p34(cdc2) protein is the catalytic subunit of the M phase-promoting factor (MPF) which is intracellular factor responsible for entry into M phase. Three mechanisms are responsible for the activitation of MPF at the onset of mitosis: binding of p34(cdc2) to cyclin B, a series of phosphorylation/dephosphorylation events on the two subunits and translocation of the complex to the nucleus or microtubules.
  • Antibody come from
    Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with recombinant human p34.cdc2 fusion protein and mouse myeloma cells.
  • Other description
    Provided as solution in phosphate buffered saline with 0.08% sodium azide. Protein A/G Chromatography
  • Clone
    POH-1
  • Isotype
    IgG2a
  • Antigen antibody binding interaction
    Mouse anti p34 cdc-2 nuclear protein Antibody
  • Antibody is raised in
    Mouse
  • Antibody s reacts with
    Human, Bovine, Xenopus, Mouse, Rat
  • Antibody s reacts with these species
    This antibody doesn't cross react with other species
  • Label
    unlabeled
  • Antibody s specificity
    No Data Available
  • Application
    Immunohistochemistry (Paraffin Sections), Immunocytochemistry, Western Blot, Immunoprecipitation
  • Antibody s suited for
    Detects three bands within the 34 kDa region corresponding to p34 and its cleavage products. Can also be used for IHC on paraffin-embedded tissue (10 µg/ml) as well as immunocytochemistry and immunoprecipitation. Optimal concentration should be evaluated by serial dilutions.
  • Storage
    -20ºC
  • Relevant references
    1. Lukas, J., et al. Distinct forms of human CDC2 identified by novel monoclonal antibodies. Eur. J. Biochem. 1992, 207, 169-176_x000B__x000B_2. Borgne, A. & Meijer, L. Sequential dephosphorylation of p34(cdc2) on Thr-14 and Tyr-15 at the prophase/metaphase transition. J. Biol. Chem. 1996, 271, 27847-27854_x000B__x000B_3. Kao, G.D., et al. p34(Cdc2) kinase activity is excluded from the nucleus during the radiation-induced G2 arrest in HeLa cells. J. Biol. Chem. 1999, 274, 34779-34784
  • Protein number
    see ncbi
  • Warnings
    This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
  • Antibody s link
    Antibody_s_link
  • Gene target
    p34 cdc-2 protein
  • Host
    mouse
  • isotype filter
    • IgG2a
  • Short name
    Mouse anti p34 cdc-2 protein
  • label filter
    • unlabeled
  • technique filter
    • Mouse
    • anti
  • Technique
    Mouse, anti, antibody to, mouses
  • Alternative technique
    murine, antibodies