-
Category
Primary Antibodies
-
Long description
p34(Cdc2) kinase is a an essential nuclear protein for mitosis in human cells. During the prophase/metaphase transition the inactive phosphorylated form of p34(cdc2) is dephosphorylated to make the active form of the protein. This dephosphorylation is performed by the p80(cdc25) dual-specificity phosphatase. The regulatory subunit of p34(cdc2) is cyclin B, a 47-52 kDa protein. The p34(cdc2) protein is the catalytic subunit of the M phase-promoting factor (MPF) which is intracellular factor responsible for entry into M phase. Three mechanisms are responsible for the activitation of MPF at the onset of mitosis: binding of p34(cdc2) to cyclin B, a series of phosphorylation/dephosphorylation events on the two subunits and translocation of the complex to the nucleus or microtubules.
-
Antibody come from
Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with recombinant human p34.cdc2 fusion protein and mouse myeloma cells.
-
Other description
Provided as solution in phosphate buffered saline with 0.08% sodium azide. Protein A/G Chromatography
-
Clone
POH-1
-
Antigen antibody binding interaction
Mouse anti p34 cdc-2 nuclear protein Antibody
-
Antibody is raised in
Mouse
-
Antibody s reacts with
Human, Bovine, Xenopus, Mouse, Rat
-
Antibody s reacts with these species
This antibody doesn't cross react with other species
-
Antibody s specificity
No Data Available
-
Application
Immunohistochemistry (Paraffin Sections), Immunocytochemistry, Western Blot, Immunoprecipitation
-
Antibody s suited for
Detects three bands within the 34 kDa region corresponding to p34 and its cleavage products. Can also be used for IHC on paraffin-embedded tissue (10 µg/ml) as well as immunocytochemistry and immunoprecipitation. Optimal concentration should be evaluated by serial dilutions.
-
Storage
-20ºC
-
Relevant references
1. Lukas, J., et al. Distinct forms of human CDC2 identified by novel monoclonal antibodies. Eur. J. Biochem. 1992, 207, 169-176_x000B__x000B_2. Borgne, A. & Meijer, L. Sequential dephosphorylation of p34(cdc2) on Thr-14 and Tyr-15 at the prophase/metaphase transition. J. Biol. Chem. 1996, 271, 27847-27854_x000B__x000B_3. Kao, G.D., et al. p34(Cdc2) kinase activity is excluded from the nucleus during the radiation-induced G2 arrest in HeLa cells. J. Biol. Chem. 1999, 274, 34779-34784
-
Protein number
see ncbi
-
Warnings
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
-
-
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
-
Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
-
Latin name
Mus musculus
