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Mouse anti MyoD1

  • Catalog number
    X1414M
  • Product name
    Mouse anti MyoD1
  • Size
    100 μg
  • Price
    Ask For Price
  • Contact us
    Mouse anti MyoD1
  • Category
    Primary Antibodies
  • Long description
    Recognizes a protein of 45kDa, identified as MyoD1. The epitope of this antibody maps in between amino acid 3-56 in the N-terminus of mouse MyoD1 protein. It does not cross react with myogenin, Myf5, or Myf6. Occassionally nuclear expression/staining of MyoD1 is seen in ectomesenchymoma and a subset of WilmÕs tumors. Weak cytoplasmic staining/presence is observed in several non-muscle tissues, including glandular epithelium and also in rhabdomyosarcomas, neuroblastomas, EwingÕs sarcomas and alveolar soft part sarcomas. The 5.2F antibody to MyoD1 labels the nuclei of myoblasts in developing muscle tissues. MyoD1 is not detected in normal adult tissue. Occassionally nuclear expression of MyoD1 is seen in ectomesenchymoma and a subset of WilmÕs tumors.
  • Antibody come from
    Hybridoma produced by the fusion of splenocytes from mice immunized with recombinant protein corresponding to amino acids 523-768 of human CAP-E and mouse myeloma cells.
  • Other description
    Provided as solution in phosphate buffered saline with 0.08% sodium azide. Protein A/G Chromatography
  • Clone
    5.2F
  • Isotype
    IgG2a
  • Antigen antibody binding interaction
    Mouse anti MyoD1 Antibody
  • Antibody is raised in
    Mouse
  • Antibody s reacts with
    Human, Mouse, Rat, Chicken
  • Antibody s reacts with these species
    This antibody doesn't cross react with other species
  • Label
    unlabeled
  • Antibody s specificity
    No Data Available
  • Application
    ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry (Frozen and Paraffn Sections), Immunofluorescense
  • Antibody s suited for
    This antibody can be used for Western blotting (1-5 µg/ml). Optimal concentration should be evaluated by serial dilutions.
  • Storage
    -20ºC
  • Relevant references
    1. Hirano, T.R., et al. 'Condensins, chromosome condensation protein complexes containing XCAP-C, XCAP-E and a Xenopus homolog of the Drosphila Barren protein.' Cell, 1997, 89, 511-521._x000B__x000B_2. Schmiesing, J.A., et al. 'A human condensin complex containing hCAP-C, hCAP-E and CNAP1, a homolog of Xenopus XCAP-D2, colocalizes with phosphorylated histone H3 during the early stage of mitotic chromosome condensation.' Mol. Cell Biol. 2000, 20, 6996-7006
  • Protein number
    see ncbi
  • Warnings
    This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
  • Antibody s link
    Antibody_s_link
  • Alternative to gene target
    • [ "myogenic differentiation 1", "bHLHc1 and MYF3 and MYOD and PUM", "MYOD1 and IDBG-34281 and ENSG00000129152 and 4654", "E-box binding", "nuclei", "Myod1 and IDBG-186053 and ENSMUSG00000009471 and 17927", "MYOD1 and IDBG-632365 and ENSBTAG00000002216 and 281938" ]
  • Gene target
    MyoD1
    • Gene info
  • Gene symbol
    MYOD1
  • Host
    mouse
  • isotype filter
    • IgG2a
  • Short name
    Mouse anti MyoD1
  • label filter
    • unlabeled
  • technique filter
    • Mouse
    • anti
  • Technique
    Mouse, anti, antibody to, mouses
  • Alternative technique
    murine, antibodies