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Mouse anti Human IgG4 (pFc subclass specific)

  • Catalog number
    MAHu/IgG4
  • Product name
    Mouse anti Human IgG4 (pFc subclass specific)
  • Size
    0,5 mg
  • Category
    Secondary Antibodies
  • Long description
    The reactivity of the antiserum is restricted to a subclass specific determinant on the pFc portion of the IgG4 molecule as tested in indirect binding enzyme immunoassay, immunoblotting, immunoprecipitation and indirect immunoperoxidase staining of cytoplasmic Ig. To identify the presence of IgG4 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, indirect immunoperoxidase and indirect immunofluorescence staining of cytoplasmic IgG4, and immunoblotting. The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:50 to 1:200; in ELISA from 1:500 upwards; in Western blotting from 1:1,000 upwards. These data should be interpreted as general recommendations only.`
  • Antibody come from
    Highly purified monoclonal IgG4 isolated from pooled human serum
  • Other description
    Delipidated, heat inactivated lyophilized stable ascites No preservative added, as it may interfere with the antibody activity. No foreign protein added. IgG concentration is 1.0 mg/ml. No foreign proteins added.
  • Clone
    NI 315 (HP 6206)
  • Isotype
    IgG1
  • Antigen antibody binding interaction
    Mouse anti Human IgG4 (pFc subclass specific) Antibody
  • Antibody is raised in
    Mouse
  • Antibody s reacts with
    Human
  • Antibody s reacts with these species
    The antiSerum does not react with any other component of the Human Ig system or any other Human plasma protein as tested. This antiSerum has not been tested for cross-reactivity with other species.
  • Label
    unlabeled
  • Antibody s specificity
    Monoclonal Mouse antibody to Human IgG4, subclass specific.
  • Research interest
    Immunology
  • Application
    Indirect immunofluorescence,ELISA
  • Antibody s suited for
    Indirect immunofluorescence,ELISA.
  • Storage
    The lyophilized product is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Reconstitute the lyophilized product by adding 0.5 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted ascites should be stored at +4ºC, not refrozen, and preferably used the same day. Lyophilized at +4° C--at least 10 years. Reconstituted at or below -20° C--3-5 years. Reconstituted at +4° C--7 days
  • Relevant references
    no information yet
  • Protein number
    see ncbi
  • Warnings
    This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
  • Antibody s link
    Antibody_s_link
  • Gene target
    IgG4 pFc subclass specific
  • Host
    mouse
  • isotype filter
    • IgG1
  • Short name
    Mouse anti IgG4 (pFc subclass specific)
  • label filter
    • unlabeled
  • technique filter
    • anti human
    • Mouse
    • anti
  • Technique
    anti human, Mouse, anti, antibody to, anti human antibodies, mouses
  • Alternative technique
    murine, antibodies