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Category
Primary Antibodies
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Long description
CNTF is a survival factor for various neuronal cell types. Seems to prevent the degeneration of motor axons after axotomy. CNTF is a polypeptide hormone and nerve growth factor whose actions have mainly been studied in the nervous system where it promotes neurotransmitter synthesis and neurite outgrowth in certain neuronal populations including astrocytes. The protein is a potent survival factor for neurons and oligodendrocytes and may be relevant in reducing tissue destruction during inflammatory attacks. A mutation in this gene, which results in aberrant splicing, leads to ciliary neurotrophic factor deficiency, but this phenotype is not causally related to neurologic disease. In addition to the predominant monocistronic transcript originating from this locus, the gene is also co-transcribed with the upstream ZFP91 gene. Co-transcription from the two loci results in a transcript that contains a complete coding region for the zinc finger protein but lacks a complete coding region for ciliary neurotrophic factor. CNTF has also been shown to be expressed by cells on the bone surface, and to reduce the activity of bone forming cells, osteoblasts.
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Antibody come from
Human recombinant ciliary neurotrophic factor (hCNTF).
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Other description
Provided as solution in phosphate buffered saline with 0.08% sodium azide. Protein A/G Chromatography
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Clone
AS23
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Antigen antibody binding interaction
Mouse anti Ciliary Neurotrophic Factor (CNTF) Antibody
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Antibody is raised in
Mouse
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Antibody s reacts with
Human
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Antibody s reacts with these species
This antibody doesn't cross react with other species
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Antibody s specificity
No Data Available
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Application
Western Blot, Immunoprecipitation, Enzyme Immunoassay
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Antibody s suited for
Applications: ELISA: This antibody has been used as a coating antibody at 10 ug/ml in a two site ELISA with PS2-B. Typically the ELISA has a working range of 0-20000 pg/ml and a sensitivity of 10 pg/ml when used with biotin conjugated CNTF antibody (Cat. No. L120P). Recommended sample volumes are 25-200 µl. Recovery of hCNTF in human plasma is typically > 85% in the ELISA (Kenney, JS, Masada, MP, and Allison AC, Laboratory Methods in Immunology, Vol. I, 231, 1990). Western Blot: This antibody can be used at a range from1 to 5 µg/ml to visualize hCNTF in Western Blots with appropriate secondary reagents. Immunoprecipitation: This antibody has been used to immunoprecipitate > 90% hCNTF in solution using a double-antibody method. (Midgley, AR and Hepburn, MR, Methods Enzymol., 70, 266, 1980).
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Storage
-20ºC
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Relevant references
1. Lam, A., et al. 'Sequence and structural organization of the human gene encoding ciliary neurotrophic factor.' Gene, 1991, 102, 271-276_x000B__x000B_2. Cargill, M., et al. 'Characterization of single-nucleotide polymorphisms in coding regions of human genes.' Nat Genet 1999, 22, 231-238
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Protein number
see ncbi
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Warnings
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Aplha, transcription related growth factors and stimulating factors or repressing nuclear factors are complex subunits of proteins involved in cell differentiation. Complex subunit associated factors are involved in hybridoma growth, Eosinohils, eritroid proliferation and derived from promotor binding stimulating subunits on the DNA binding complex. NFKB 105 subunit for example is a polypetide gene enhancer of genes in B cells.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
