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Mouse anti Ciliary Neurotrophic Factor (CNTF)

  • Catalog number
    L100M
  • Product name
    Mouse anti Ciliary Neurotrophic Factor (CNTF)
  • Size
    500 μg
  • Category
    Primary Antibodies
  • Long description
    CNTF is a survival factor for various neuronal cell types. Seems to prevent the degeneration of motor axons after axotomy. CNTF is a polypeptide hormone and nerve growth factor whose actions have mainly been studied in the nervous system where it promotes neurotransmitter synthesis and neurite outgrowth in certain neuronal populations including astrocytes. The protein is a potent survival factor for neurons and oligodendrocytes and may be relevant in reducing tissue destruction during inflammatory attacks. A mutation in this gene, which results in aberrant splicing, leads to ciliary neurotrophic factor deficiency, but this phenotype is not causally related to neurologic disease. In addition to the predominant monocistronic transcript originating from this locus, the gene is also co-transcribed with the upstream ZFP91 gene. Co-transcription from the two loci results in a transcript that contains a complete coding region for the zinc finger protein but lacks a complete coding region for ciliary neurotrophic factor. CNTF has also been shown to be expressed by cells on the bone surface, and to reduce the activity of bone forming cells, osteoblasts.
  • Antibody come from
    Human recombinant ciliary neurotrophic factor (hCNTF).
  • Other description
    Provided as solution in phosphate buffered saline with 0.08% sodium azide. Protein A/G Chromatography
  • Clone
    AS23
  • Isotype
    IgG1
  • Antigen antibody binding interaction
    Mouse anti Ciliary Neurotrophic Factor (CNTF) Antibody
  • Antibody is raised in
    Mouse
  • Antibody s reacts with
    Human
  • Antibody s reacts with these species
    This antibody doesn't cross react with other species
  • Label
    unlabeled
  • Antibody s specificity
    No Data Available
  • Application
    Western Blot, Immunoprecipitation, Enzyme Immunoassay
  • Antibody s suited for
    Applications: ELISA: This antibody has been used as a coating antibody at 10 ug/ml in a two site ELISA with PS2-B. Typically the ELISA has a working range of 0-20000 pg/ml and a sensitivity of 10 pg/ml when used with biotin conjugated CNTF antibody (Cat. No. L120P). Recommended sample volumes are 25-200 µl. Recovery of hCNTF in human plasma is typically > 85% in the ELISA (Kenney, JS, Masada, MP, and Allison AC, Laboratory Methods in Immunology, Vol. I, 231, 1990). Western Blot: This antibody can be used at a range from1 to 5 µg/ml to visualize hCNTF in Western Blots with appropriate secondary reagents. Immunoprecipitation: This antibody has been used to immunoprecipitate > 90% hCNTF in solution using a double-antibody method. (Midgley, AR and Hepburn, MR, Methods Enzymol., 70, 266, 1980).
  • Storage
    -20ºC
  • Relevant references
    1. Lam, A., et al. 'Sequence and structural organization of the human gene encoding ciliary neurotrophic factor.' Gene, 1991, 102, 271-276_x000B__x000B_2. Cargill, M., et al. 'Characterization of single-nucleotide polymorphisms in coding regions of human genes.' Nat Genet 1999, 22, 231-238
  • Protein number
    see ncbi
  • Warnings
    This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
  • Antibody s link
    Antibody_s_link
  • Alternative to gene target
    • [ "ciliary neurotrophic factor", "HCNTF", "CNTF and IDBG-409278 and ENSG00000242689 and 1270", "growth factor activity", "Extracellular", "Cntf and IDBG-254935 and ENSMUSG00000079415 and 12803", "CNTF and IDBG-637978 and ENSBTAG00000003624 and 100848228" ]
  • Gene target
    Ciliary Neurotrophic Factor CNTF
    • Gene info
    Gene info
    Locus Specific Databases:ALSOD, the Amyotrophic Lateral Sclerosis Online Genetic Database
  • Gene symbol
    ZFP91-CNTF, CNTF
  • Host
    mouse
  • isotype filter
    • IgG1
  • Short name
    Mouse anti Ciliary Neurotrophic Factor (CNTF)
  • label filter
    • unlabeled
  • technique filter
    • Mouse
    • anti
  • Technique
    Mouse, anti, antibody to, mouses
  • Alternative technique
    murine, antibodies