• Catalog number
    0272
  • Product name
    Mouse anti CD27 antibody conjugated to FITC
  • Size
    100 Tests
  • Category
    Primary Antibodies
  • Long description
    The CD27 antigen recognizes disulfide-linked dimer nerve growth factor (NGF) super family of cells expressing the 55kD surface antigen. CD27 acts as a co-stimulatory on T lymphocytes with its ligand of CD70.
  • Antibody come from
    n/a
  • Other description
    Provided as sterile filtered solution in phosphate buffered saline with 0.08% sodium azide and 0.2% carrier protein. Protein A/G Chromatography
  • Clone
    DEN-3
  • Isotype
    IgG1
  • Antigen antibody binding interaction
    Mouse anti CD27 antibody conjugated to FITC Antibody
  • Antibody is raised in
    Mouse
  • Antibody s reacts with
    Human
  • Antibody s reacts with these species
    This antibody doesn't cross react with other species
  • Label
    FITC
  • Antibody s specificity
    No Data Available
  • Research interest
    CD Marker
  • Application
    Flow Cytometry
  • Antibody s suited for
    PBMC: Add10 µl of MAB/10^6 PBMC in 100 µl PBS. Mix gently and incubate for 15 minutes at 2 to 8¼C. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add 10 µl of MAB/100 µl of whole blood. Mix gently and incubate for 15 minutes at room temperature (20¼C). Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturerÕs instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.
  • Storage
    4ºC
  • Relevant references
    1. Morimoto C., Cluster report : CD27. In Schlossman SF., Boumsell L., Gilks W., et al., eds. Leukocyte Typing V: White Cell Differentiation Antigens. Oxford: Oxford University Press: 1995:356-357. _x000B__x000B_2. CD27 induction on thymocytes. J Immunol 1990 Sep ;145(5):1356-63 Martorell J; Rojo I; Vilella R; Martinez-Caceres E; Vives J _x000B__x000B_3. CD27 expression by a distinct subpopulation of human B lymphocytes. Eur J Immunol 1990 De;20(12):2679-84 Maurer D; Holter W; Majdic O; Fischer GF; Knapp W _x000B__x000B_4. Regulation of expression of CD27, a T cell-specific member of a novel family of membrane receptors. J Immunol 1991 Apr 1;146(8):2488-94 de Jong R; Loenen WA; Brouwer M; van Emmerik L; de Vries EF; Borst J; van Lier RA _x000B__x000B_5. The T-cell activation antigen CD27 is a member of the growth factor/tumor necrosis factor receptor gene family. Camerini D., Walz G., Loenen W., Borst J., Seed B. J. Immunol. 1991:147(9):3165-3169
  • Protein number
    see ncbi
  • Warnings
    This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
  • Antibody s link
  • Alternative to gene target
    • [ "CD27 molecule", "S152 and S152. LPFS2 and T14 and TNFRSF7 and Tp55", "CD27 and IDBG-14014 and ENSG00000139193 and 939", "cysteine-type endopeptidase inhibitor activity involved in apoptotic process", "Extracellular", "Cd27 and IDBG-189750 and ENSMUSG00000030336 and 21940", "CD27 and IDBG-640494 and ENSBTAG00000014725 and 512514" ]
  • Gene target
    CD27 conjugated
  • Gene info
    Gene info
  • Gene symbol
    CD27-AS1, CD27
  • Host
    mouse
  • isotype filter
    • IgG1
  • Short name
    Mouse anti CD27 antibody conjugated FITC
  • label filter
    • FITC
  • technique filter
    • Antibody
    • Mouse
    • anti
    • FITC
  • Technique
    Antibody, Mouse, anti, FITC, antibody to, antibodies against human proteins, antibodies for, antibody Conjugates, Fluorescein, mouses
  • Alternative technique
    antibodies, murine, fluorescine