gentaur logogentaur logo
x

Goat anti Rat IgG1 IgG2a IgG2b IgG2c IgA IgM (heavy and light chains) (non Human and Mouse)

  • Catalog number
    GARa/mIg/7S
  • Product name
    Goat anti Rat IgG1 IgG2a IgG2b IgG2c IgA IgM (heavy and light chains) (non Human and Mouse)
  • Size
    1 mL
  • Category
    Secondary Antibodies
  • Long description
    The defined antibody specificity is directed to the major isotypes of the rat immunoglobulin system (classes, subclasses and L chain types) including antibody to common determinants, to class and subclass-specific determinants, and to the surface determinants of the common Fab portion as tested against as tested in immunoelectrophoresis and double radial immunodiffusion (Ouchterlony). The cytochemical grade allows the use in different types of highly sensitive immunoassays on appropriately treated cell and tissue substrates; in radioimmunoassay; for the production of immunoconjugates with a selected marker; to prepare immunoaffinity adsorbents by coupling to an artificial carrier; in non-isotopic methodology based on solid phase immunochemistry (e.g. ELISA), both as catching antibody and detection reagent; in Western blotting. This product is not pre-diluted. The optimum working dilution of each product should be established by titration before being used. Working dilutions for histochemical and cytochemical use are usually between 1:50 and 1:250; in ELISA and comparable non-precipitating antibody-binding assays are between 1:500 and 1:5,000.
  • Antibody come from
    Highly purified IgG, including all subclasses, and pools of homogenous IgA and IgM isolated from rat serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
  • Other description
    Purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity.
  • Clone
    Polyclonal
  • Isotype
    not specified, IgA, IgG1, IgG2a, IgG2b, IgG2c, IgM
  • Antigen antibody binding interaction
    Goat anti Rat IgG1 IgG2a IgG2b IgG2c IgA IgM (heavy and light chains) (non Human and Mouse) Antibody
  • Antibody is raised in
    Goat
  • Antibody s reacts with
    Rat
  • Antibody s reacts with these species
    Inter-species cross-reactivity is a normal feature of antibodies to mammalian immunoglobulins, since homologous proteins of different species frequently share antigenic determinants. The degree of cross-reactivity is also dependent on the concentRations of the reactants and the sensitivity of the assay arrangement. If the product is to be used in the presence of material originating from an other species than Human and Mouse, prior screening for cross-reactivity with that material is essential and additional adsorption of the reagent may be required to ensure intra-assay specificity.
  • Label
    unlabeled
  • Antibody s specificity
    Purified IgG fraction of polyclonal Goat antiSerum to Rat immunoglobulins IgG, IgA, IgM, heavy and light chains, adsorbed for Human and Mouse cross-reactivity.
  • Research interest
    Veterinary
  • Application
    Indirect immunofluorescence,ELISA,Dot blot,Immunoblotting
  • Antibody s suited for
    Indirect immunofluorescence,ELISA,Dot blot,Immunoblotting.
  • Storage
    The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. It is reconstituted by adding 1 ml sterile distilled water. Working dilutions are prepared by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product. Lyophilized at +4° C--at least 10 years. Reconstituted at or below -20° C--3-5 years. Reconstituted at +4° C--7 days
  • Relevant references
    no information yet
  • Protein number
    see ncbi
  • Warnings
    This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
  • Antibody s link
    Antibody_s_link
  • Gene target
    IgG1 IgG2a IgG2b IgG2c heavy light chains non
  • Host
    Goat, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial.
  • isotype filter
    • not specified
  • Short name
    Goat anti IgG1 IgG2a IgG2b IgG2c IgA IgM (heavy light chains) (non Mouse)
  • label filter
    • unlabeled
  • technique filter
    • light
    • Mouse
    • anti
    • Goat
    • IgA
    • IgM
  • Technique
    light, Mouse, anti, Goat, IgA, IgM, antibody to, goats, IgAs, IgMs, mouses
  • Alternative technique
    murine, antibodies, caprine, immunoglobulin-a, immunoglobulin-m