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Goat anti Rat albumin antibody conjugated with Horseradish peroxidase

  • Catalog number
    GARa/Alb/PO
  • Product name
    Goat anti Rat albumin antibody conjugated with Horseradish peroxidase
  • Size
    1 mL
  • Category
    Primary Antibodies
  • Long description
    Tested in immunoelectrophoresis and double radial immunodiffusion against pooled normal rat serum and purified rat albumin. One characteristic precipitin line is obtained against pooled normal rat serum using different antigen/antibody concentration ratio’s. Precipitin lines against normal rat serum and purified rat albumin give a reaction of full identity. In enzyme-immunocytochemical and immunohistochemical staining for the detection of albumin, of appropriately treated cell and tissue substrates at the cellular and subcellular level. In non-isotopic assay methodology (e.g. ELISA) to identify and measure albumin in rat serum or other body fluid. In electron microscopy, since the complex between the conjugated antibody and the antigen also has electron-dense properties. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions for histochemical and cytochemical use are usually between 1:100 and 1:500; in ELISA and comparable non-precipitating antibody-binding assays between 1:1,000 and 1:10,000.
  • Antibody come from
    Purified albumin isolated from pooled rat serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
  • Other description
    Peroxidase-coupled purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity.
  • Clone
    Polyclonal
  • Isotype
    not specified
  • Antigen antibody binding interaction
    Goat anti Rat albumin antibody conjugated with Horseradish peroxidase Antibody
  • Antibody is raised in
    Goat
  • Antibody s reacts with
    Rat
  • Antibody s reacts with these species
    Inter-species cross-reactivity is a normal feature of antibodies to mammalian Serum proteins, since homologous proteins of different species frequently share antigenic determinants. The degree of cross-reactivity is also dependent on the concentRations of the reactants and the sensitivity of the assay arrangement. This antiSerum fraction has been tested for cross-reactivity by double radial immunodiffusion anti several species sera with the following results: Bovine - Goat - Human + Rabbit ± Chicken - Guinea Pig ++ Mouse ++ Sheep - Dog + Horse + Monkey + Swine + A negative reaction in double radial immunodiffusion does not exclude a weak positive reaction in more sensitive techniques.
  • Label
    HRP
  • Antibody s specificity
    Horseradish peroxidase–conjugated IgG fraction of polyclonal Goat antiSerum to Rat albumin.
  • Research interest
    Veterinary
  • Application
    ELISA,Immunocytochemistry,Immunohistochemistry (paraffin),Dot blot,Immunoblotting
  • Antibody s suited for
    ELISA,Immunocytochemistry,Immunohistochemistry (paraffin),Dot blot,Immunoblotting.
  • Storage
    Lyophilized at +4° C--at least 10 years. Reconstituted at or below -20° C--3-5 years. Reconstituted at +4° C--7 days
  • Relevant references
    no information yet
  • Protein number
    see ncbi
  • Warnings
    This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
  • Antibody s link
    Antibody_s_link
  • Gene target
    albumin conjugated with Horseradish
  • Host
    Goat, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial.
  • isotype filter
    • not specified
  • Short name
    Goat anti albumin antibody conjugated with Horseradish
  • label filter
    • HRP
  • technique filter
    • Antibody
    • anti
    • Goat
  • Technique
    Antibody, anti, Goat, antibody to, antibodies against human proteins, antibodies for, antibody Conjugates, goats
  • Alternative technique
    antibodies, caprine