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Goat anti Rabbit IgG IgA IgM (heavy and light chains) antibody conjugated with FITC

  • Catalog number
    GAR/Ig/FITC
  • Product name
    Goat anti Rabbit IgG IgA IgM (heavy and light chains) antibody conjugated with FITC
  • Size
    1 mL
  • Category
    Secondary Antibodies
  • Long description
    The reactivity of the antiserum is directed to the major isotypes of the rabbit immunoglobulin system (classes and both light chain types) including antibodies to common determinants, to class and to the surface determinants of the common Fab portion, as tested by immunoelectrophoresis and double radial immunodiffusion against pooled serum and purified immunoglobulins. In immunoelectrophoresis and double radial immunodiffusion using various antiserum concentrations against normal rabbit plasma and serum, the characteristic IgG, IgA and IgM precipitin lines are obtained. Direct immunofluorescence staining of cytoplasmic Ig of appropriately treated cell and tissue substrates; to demonstrate immunoglobulins or specific antibodies in cells and tissues; to identify circulating antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen or immune complex using a reference antibody of rabbit origin in the middle layer of the indirect test procedure. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions are usually between 1:20 and 1:80.
  • Antibody come from
    Purified polyclonal rabbit IgG, and IgA and IgM containing factions isolated from rabbit serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
  • Other description
    FITC-coupled purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity.
  • Clone
    Polyclonal
  • Isotype
    not specified, IgA, IgG, IgM
  • Antigen antibody binding interaction
    Goat anti Rabbit IgG IgA IgM (heavy and light chains) antibody conjugated with FITC Antibody
  • Antibody is raised in
    Goat
  • Antibody s reacts with
    Rabbit
  • Antibody s reacts with these species
    Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this product has not been tested in detail.
  • Label
    FITC
  • Antibody s specificity
    Fluorescein isothiocyanate–conjugated IgG fraction of polyclonal Goat antiSerum to Rabbit IgG, IgA and IgM, heavy and light chains.
  • Research interest
    Veterinary
  • Application
    ELISA,Immunocytochemistry,Immunohistochemistry (frozen),(In)direct immunofluorescence
  • Antibody s suited for
    ELISA,Immunocytochemistry,Immunohistochemistry (frozen),(In)direct immunofluorescence.
  • Storage
    The lyophilized conjugate is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. It is reconstituted by adding 1 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate. Lyophilized at +4° C--at least 10 years. Reconstituted at or below -20° C--3-5 years. Reconstituted at +4° C--7 days
  • Relevant references
    no information yet
  • Protein number
    see ncbi
  • Warnings
    This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
  • Antibody s link
    Antibody_s_link
  • Gene target
    heavy light chains conjugated with
  • Host
    Goat, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial. Rabbits
  • isotype filter
    • not specified
  • Short name
    Goat anti Rabbit IgG IgA IgM (heavy light chains) antibody conjugated with FITC
  • label filter
    • FITC
  • technique filter
    • Antibody
    • Rabbit
    • light
    • anti
    • FITC
    • Goat
    • IgA
    • IgG
    • IgM
  • Technique
    Antibody, Rabbit, light, anti, FITC, Goat, IgA, IgG, IgM, antibody to, antibodies against human proteins, antibodies for, antibody Conjugates, Fluorescein, goats, IgAs, IgGs, IgMs
  • Alternative technique
    antibodies, rabbit-anti, caprine, fluorescine, immunoglobulin-a, immunoglobulins, immunoglobulin-m