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Goat anti Mouse IgA (Fc specific)

  • Catalog number
    GAM/IgA(Fc)
  • Product name
    Goat anti Mouse IgA (Fc specific)
  • Size
    1 mL
  • Category
    Secondary Antibodies
  • Long description
    The reactivity of the antiserum is restricted to the Fc part of the IgA molecule. In immunoelectrophoresis and radial immunodiffusion, using various antiserum concentrations against normal mouse serum a single precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified IgA. No precipitation reaction is obtained with purified IgG, IgM, and IgG/Fab fragments. In precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence of IgA in mouse serum or other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of mouse IgA from serum or plasma. This antiserum is not intended for use in non-precipitating antibody-binding or other highly sensitive assays. For such use labelled and unlabelled cytochemical and immunoassay grade reagents meeting the necessary additional specificity and performance requirements are available.
  • Antibody come from
    Highly purified pools of monoclonal IgA isolated from mouse serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
  • Other description
    Delipidated, heat inactivated, lyophilized, stable whole antiserum No preservative added Total protein and IgG concentrations in the antiserum are comparable to those of pooled normal goat serum. No foreign proteins added.
  • Clone
    Polyclonal
  • Isotype
    not specified, IgA
  • Antigen antibody binding interaction
    Goat anti Mouse IgA (Fc specific) Antibody
  • Antibody is raised in
    Goat
  • Antibody s reacts with
    Mouse
  • Antibody s reacts with these species
    The antiSerum does not cross-react with any other component of the Mouse immunoglobulin system. Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail.
  • Label
    unlabeled
  • Antibody s specificity
    Precipitating polyclonal Goat antiSerum to Mouse IgA, Fc specific
  • Research interest
    Veterinary
  • Application
    Immunoprecipitation
  • Antibody s suited for
    Immunoprecipitation. In immunoelectrophoresis use 2 μl serum or equivalent against 120 μl antiserum. In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 μl antiserum in 3 mm diameter center well and 2 μl serum samples (neat and serially diluted in 2 mm diameter peripheral wells. Precipitin titre 1:16 when tested against pooled normal mouse serum in agar-block immunodiffusion titration.
  • Storage
    The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Reconstitute the lyophilized antiserum by adding 1 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4°C, not refrozen, and preferably used the same day. Lyophilized at +4° C--at least 10 years. Reconstituted at or below -20° C--3-5 years. Reconstituted at +4° C--7 days
  • Relevant references
    no information yet
  • Protein number
    see ncbi
  • Warnings
    This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
  • Antibody s link
    Antibody_s_link
  • Gene target
    Fc specific
  • Host
    Goat, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial.
  • isotype filter
    • not specified
  • Short name
    Goat anti Mouse IgA (Fc specific)
  • label filter
    • unlabeled
  • technique filter
    • anti mouse
    • Mouse
    • anti
    • Goat
    • IgA
    • FC
  • Technique
    anti mouse, Mouse, anti, Goat, IgA, FC, antibody to, anti mouse antibodies, goats, IgAs, mouses
  • Alternative technique
    murine, antibodies, caprine, immunoglobulin-a