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Goat anti Human Ig lambda light chain (free and bound) antibody conjugated with TRITC

  • Catalog number
    GAHu/BJL(SD+HD)/TRITC
  • Product name
    Goat anti Human Ig lambda light chain (free and bound) antibody conjugated with TRITC
  • Size
    1 mL
  • Category
    Secondary Antibodies
  • Long description
    The reactivity of the antiserum is directed to the surface and hidden determinants of Ig lambda light chain. In immunoelectrophoresis this antiserum is reacting with polyclonal and monoclonal immunoglobulins of the lambda type, Bence Jones proteins as well as free light chains of the lambda type. This antiserum does not react with any other protein of human serum or plasma. This conjugate is intended for use in direct immunofluorescence for the detection of polyclonal immunoglobulins, purified monoclonal immunoglobulins of the lambda type as well as free light chains or Bence Jones proteins of the lambda type in serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions are usually between 1:10 and 1:40.
  • Antibody come from
    A pool of purified Bence Jones lambda proteins isolated from human urine. Freund’s complete adjuvant is used in the first step of the immunization procedure.
  • Other description
    Fluorochrome coupled purified goat IgG lyophilized from a solution in phosphate buffered saline (pH 7.2). No preservative added, as it may interfere with the antibody activity
  • Clone
    Polyclonal
  • Isotype
    not specified
  • Antigen antibody binding interaction
    Goat anti Human Ig lambda light chain (free and bound) antibody conjugated with TRITC Antibody
  • Antibody is raised in
    Goat
  • Antibody s reacts with
    Human
  • Antibody s reacts with these species
    Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins and their fragments, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail.
  • Label
    TRITC
  • Antibody s specificity
    Tetramethylrhodamine isothiocyanate-conjugated IgG fraction of polyclonal Goat antiSerum to free and bound Human Ig lambda light chain
  • Research interest
    Immunology
  • Application
    ELISA,Immunocytochemistry,Immunohistochemistry (frozen),(In)direct immunofluorescence
  • Antibody s suited for
    ELISA,Immunocytochemistry,Immunohistochemistry (frozen),(In)direct immunofluorescence.
  • Storage
    The lyophilized product is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. It is reconstituted by adding 1 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product. Lyophilized at +4° C--at least 10 years. Reconstituted at or below -20° C--3-5 years. Reconstituted at +4° C--7 days
  • Relevant references
    no information yet
  • Protein number
    see ncbi
  • Warnings
    This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
  • Antibody s link
    Antibody_s_link
  • Gene target
    Ig lambda light chain free bound conjugated with TRITC
  • Host
    Goat, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial.
  • isotype filter
    • not specified
  • Short name
    Goat anti Ig lambda light chain (free bound) antibody conjugated with TRITC
  • label filter
    • TRITC
  • technique filter
    • anti human
    • Antibody
    • light
    • anti
    • Goat
  • Technique
    anti human, Antibody, light, anti, Goat, antibody to, anti human antibodies, antibodies against human proteins, antibodies for, antibody Conjugates, goats
  • Alternative technique
    antibodies, caprine