- Catalog numberGAGp/IgM(Fc)/Bio
- Product nameGoat anti Guinea Pig IgM (Fc specific) antibody conjugated with Biotin
- Size1 mL
- PriceAsk For Price
- CategorySecondary Antibodies
- Long descriptionThe reactivity of the antiserum is directed to the Fc subunit of the IgM molecule which expresses strict isotypic (class) specificity. It does not react with any non-Ig protein in guinea pig serum, as tested by immunoelectrophoresis and double radial immunodiffusion. In immunocytochemical and immunohistochemical use for the detection of IgM at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using an reference antibody of goat origin known to be of the IgM isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgM in guinea pig serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions for histochemical and cytochemical use are usually between 1:100 and 1:250; in ELISA and comparable non-precipitating antibody-binding assays between 1:1,000 and 1:5,000.
- Antibody come fromPurified normal IgM isolated from pooled guinea pig serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
- Other descriptionBiotin-coupled purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity.
- ClonePolyclonal
- Isotypenot specified, IgM
- Antigen antibody binding interactionGoat anti Guinea Pig IgM (Fc specific) antibody conjugated with Biotin Antibody
- Antibody is raised inGoat
- Antibody s reacts withGuinea Pig
- Antibody s reacts with these speciesInter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail.
- LabelBiotin
- Antibody s specificityBiotin-conjugated IgG fraction of polyclonal Goat antiSerum to Guinea Pig IgM, Fc specific
- Research interestVeterinary
- ApplicationELISA,Immunocytochemistry,Immunohistochemistry (paraffin),Dot blot,Immunoblotting
- Antibody s suited forELISA,Immunocytochemistry,Immunohistochemistry (paraffin),Dot blot,Immunoblotting.
- StorageThe lyophilized conjugate is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. It is reconstituted by adding 1 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate. Lyophilized at +4° C--at least 10 years. Reconstituted at or below -20° C--3-5 years. Reconstituted at +4° C--7 days
- Relevant referencesno information yet
- Protein numbersee ncbi
- WarningsThis product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
- Antibody s link
- Gene targetFc specific conjugated with
- HostGoat, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial.
- isotype filter
- not specified
- Short nameGoat anti IgM (Fc specific) antibody conjugated with
- label filter
- Biotin
- technique filter
- Antibody
- anti
- Goat
- IgM
- FC
- TechniqueAntibody, anti, Goat, IgM, FC, antibody to, antibodies against human proteins, antibodies for, antibody Conjugates, goats, IgMs
- Alternative techniqueantibodies, caprine, immunoglobulin-m