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Goat anti Guinea Pig IgG (subclass specific) antibody conjugated with FITC

  • Catalog number
    GAGp/IgG1/FITC
  • Product name
    Goat anti Guinea Pig IgG (subclass specific) antibody conjugated with FITC
  • Size
    1 mL
  • Category
    Secondary Antibodies
  • Long description
    The reactivity of the antiserum is directed to the subclass IgG1. It does not react with IgG2, IgG/Fab fragments, IgM and IgA or any non-Ig protein in guinea pig serum, as tested by immunoelectrophoresis and double radial immunodiffusion. To identify and measure IgG1, antigen or antibody, at the cellular and subcellular level by immunofluorescence staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen or immune complex using a reference antibody of guinea pig origin in the middle layer of the indirect test procedure. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions are usually between 1:20 and 1:80.
  • Antibody come from
    Pools of highly purified homogenous IgG1 isolated from guinea pig serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
  • Other description
    Fluorochrome-coupled purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity.
  • Clone
    Polyclonal
  • Isotype
    not specified, IgG
  • Antigen antibody binding interaction
    Goat anti Guinea Pig IgG (subclass specific) antibody conjugated with FITC Antibody
  • Antibody is raised in
    Goat
  • Antibody s reacts with
    Guinea Pig
  • Antibody s reacts with these species
    Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail.
  • Label
    FITC
  • Antibody s specificity
    Fluorescein isothiocyanate-conjugated IgG fraction of polyclonal Goat antiSerum to Guinea Pig IgG1, subclass specific
  • Research interest
    Veterinary
  • Application
    ELISA,Immunocytochemistry,Immunohistochemistry (frozen),(In)direct immunofluorescence
  • Antibody s suited for
    ELISA,Immunocytochemistry,Immunohistochemistry (frozen),(In)direct immunofluorescence.
  • Storage
    The lyophilized conjugate is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. It is reconstituted by adding 1 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate. Lyophilized at +4° C--at least 10 years. Reconstituted at or below -20° C--3-5 years. Reconstituted at +4° C--7 days
  • Relevant references
    no information yet
  • Protein number
    see ncbi
  • Warnings
    This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
  • Antibody s link
    Antibody_s_link
  • Gene target
    subclass specific conjugated with
  • Host
    Goat, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial.
  • isotype filter
    • not specified
  • Short name
    Goat anti IgG (subclass specific) antibody conjugated with FITC
  • label filter
    • FITC
  • technique filter
    • Antibody
    • anti
    • FITC
    • Goat
    • IgG
  • Technique
    Antibody, anti, FITC, Goat, IgG, antibody to, antibodies against human proteins, antibodies for, antibody Conjugates, Fluorescein, goats, IgGs
  • Alternative technique
    antibodies, caprine, fluorescine, immunoglobulins