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Goat anti Guinea Pig IgG (heavy and light chains)

  • Catalog number
    GAGp/IgG(H+L)/7S
  • Product name
    Goat anti Guinea Pig IgG (heavy and light chains)
  • Size
    10 mg
  • Category
    Secondary Antibodies
  • Long description
    The reactivity of the antiserum is directed to the Fc and Fab subunits of the IgG molecule. It includes a certain degree of reactivity with other immunoglobulins via the common Fab portion. It does not react with any non-Ig protein in guinea pig serum, as tested by immunoelectrophoresis and double radial immunodiffusion. As unlabelled primary or secondary reagent for indirect detection techniques, to prepare conjugates with markers of the user’s own choice, to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching or detection antibody in non-isotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical procedure or solids phase coupling technique, the optimum concentration of the IgG preparation should always be established by titration. Typical working dilutions in histochemistry are usually between 1:50 and 1:250; in ELISA and comparable non-precipitating antibody-binding assays between 1:500 and 1:5,000.
  • Antibody come from
    Purified normal IgG isolated from pooled guinea pig serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
  • Other description
    Purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity. No foreign protein added.
  • Clone
    Polyclonal
  • Isotype
    not specified, IgG
  • Antigen antibody binding interaction
    Goat anti Guinea Pig IgG (heavy and light chains) Antibody
  • Antibody is raised in
    Goat
  • Antibody s reacts with
    Guinea Pig
  • Antibody s reacts with these species
    Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail.
  • Antibody s specificity
    Purified IgG fraction of polyclonal Goat antiSerum to Guinea Pig IgG, heavy and light chains
  • Research interest
    Veterinary
  • Application
    Indirect immunofluorescence,ELISA,Dot blot,Immunoblotting
  • Antibody s suited for
    Indirect immunofluorescence,ELISA,Dot blot,Immunoblotting. Precipitin titre not less than 1:64 when tested against normal guinea pig serum in agar block titration.
  • Storage
    The lyophilized IgG fraction is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. It is reconstituted by adding 1 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product. Lyophilized at +4° C--at least 10 years. Reconstituted at or below -20° C--3-5 years. Reconstituted at +4° C--7 days
  • Relevant references
    no information yet
  • Protein number
    see ncbi
  • Warnings
    This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
  • Antibody s link
    Antibody_s_link
  • Gene target
    heavy light chains
  • Host
    Goat, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial.
  • isotype filter
    • not specified
  • Short name
    Goat anti IgG (heavy light chains)
  • technique filter
    • light
    • anti
    • Goat
    • IgG
  • Technique
    light, anti, Goat, IgG, antibody to, goats, IgGs
  • Alternative technique
    antibodies, caprine, immunoglobulins