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Goat anti Canavalia Concanavalin A antibody conjugated with Horseradish peroxidase

  • Catalog number
    GA/ConA/PO
  • Product name
    Goat anti Canavalia Concanavalin A antibody conjugated with Horseradish peroxidase
  • Size
    1 mL
  • Category
    Secondary Antibodies
  • Long description
    Tested in immunoelectrophoresis, using different antigen/antibody concentration ratio’s the antiserum gives one characteristic precipitin line. In enzyme-immunocytochemical and immunohistochemical staining of concanavalin A in appropriately prepared substrates. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. The entire test system must be free of sodium azide, since it inhibits the enzyme activity. Working dilutions may vary widely and should be determined by titration.
  • Antibody come from
    The lectin concanavalin A is a cell-agglutinating protein reacting specifically with molecules which contain alpha-D-mannopyranosyl, alpha-D-glucopyranosyl and sterically related residues. At pH 4.5-5.6 it exist as a dimer, above pH 7.0 predominantly as a tetramer. The monomer has a molecular weight of about 52,000. Freund’s complete adjuvant is used in the first step of the immunization procedure.
  • Other description
    Horseradish peroxidase-coupled purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added.
  • Clone
    Polyclonal
  • Isotype
    not specified
  • Antigen antibody binding interaction
    Goat anti Canavalia Concanavalin A antibody conjugated with Horseradish peroxidase Antibody
  • Antibody is raised in
    Goat
  • Antibody s reacts with
    Plant
  • Antibody s reacts with these species
    Inter-species cross-reactivity is a normal feature of antibodies since homologous proteins of different species frequently share antigenic determinants. The degree of cross-reactivity is also dependent on the concentRation of the reactants and the sensitivity of the assay arrangement. Cross-reactivity of this antiSerum has not been tested in detail.
  • Label
    HRP
  • Antibody s specificity
    Horseradish peroxidase-conjugated IgG fraction of polyclonal Goat antiSerum to concanavalin A from jack bean
  • Application
    ELISA,Immunocytochemistry,Immunohistochemistry (paraffin),Dot blot,Immunoblotting
  • Antibody s suited for
    ELISA,Immunocytochemistry,Immunohistochemistry (paraffin),Dot blot,Immunoblotting.
  • Storage
    The lyophilized conjugate is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. It is reconstituted by adding 1 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate. Lyophilized at +4° C--at least 10 years. Reconstituted at or below -20° C--3-5 years. Reconstituted at +4° C--7 days
  • Relevant references
    no information yet
  • Protein number
    see ncbi
  • Warnings
    This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
  • Antibody s link
    Antibody_s_link
  • Gene target
    Canavalia Concanavalin A conjugated with Horseradish
  • Host
    Goat, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial.
  • isotype filter
    • not specified
  • Short name
    Goat anti Canavalia Concanavalin A antibody conjugated with Horseradish
  • label filter
    • HRP
  • technique filter
    • Antibody
    • anti
    • Goat
  • Technique
    Antibody, anti, Goat, antibody to, antibodies against human proteins, antibodies for, antibody Conjugates, goats
  • Alternative technique
    antibodies, caprine