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Goat anti Bovine IgG (heavy and light chains) antibody conjugated with Horseradish peroxidase

  • Catalog number
    GAB/IgG(H+L)/PO
  • Product name
    Goat anti Bovine IgG (heavy and light chains) antibody conjugated with Horseradish peroxidase
  • Size
    1 mL
  • Category
    Secondary Antibodies
  • Long description
    The reactivity of the antiserum is directed to the Fc and Fab subunits of the IgG molecule. It includes some reactivity with other immunoglobulins via the common Fab portion. It does not react with any non-Ig protein in bovine serum, as tested by immunoelectrophoresis and double radial immunodiffusion. In enzyme-immunocytochemical and immunohistochemical staining for the detection of IgG, antigen or antibody, of appropriately treated cell and tissue substrates at the cellular and subcellular level. In non-isotopic assay methodology (e.g. ELISA) to identify and measure a specific IgG in bovine serum or other body fluid. In electron microscopy, since the complex between the conjugated antibody and the antigen also has electron-dense properties. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions for histochemical and cytochemical use are usually between 1:100 and 1:500; in ELISA and comparable non-precipitating antibody-binding assays between 1:1,000 and 1:4,000.
  • Antibody come from
    Purified normal IgG isolated from pooled bovine serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
  • Other description
    Peroxidase-coupled purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity.
  • Clone
    Polyclonal
  • Isotype
    not specified, IgG
  • Antigen antibody binding interaction
    Goat anti Bovine IgG (heavy and light chains) antibody conjugated with Horseradish peroxidase Antibody
  • Antibody is raised in
    Goat
  • Antibody s reacts with
    Bovine
  • Antibody s reacts with these species
    Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail, however tested in double radial immunodiffusion no reaction has been observed with Human and Swine Serum.
  • Label
    HRP
  • Antibody s specificity
    Horseradish peroxidase–conjugated IgG fraction of polyclonal Goat antiSerum to Bovine IgG, heavy and light chains
  • Research interest
    Veterinary
  • Application
    ELISA,Immunocytochemistry,Immunohistochemistry (paraffin),Dot blot,Immunoblotting
  • Antibody s suited for
    ELISA,Immunocytochemistry,Immunohistochemistry (paraffin),Dot blot,Immunoblotting.
  • Storage
    The lyophilized conjugate is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. It is reconstituted by adding 1 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate Lyophilized at +4° C--at least 10 years. Reconstituted at or below -20° C--3-5 year. Reconstituted at +4° C--7 days
  • Relevant references
    no information yet
  • Protein number
    see ncbi
  • Warnings
    This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
  • Antibody s link
    Antibody_s_link
  • Gene target
    heavy light chains conjugated with Horseradish
  • Host
    Goat, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial.
  • isotype filter
    • not specified
  • Short name
    Goat anti IgG (heavy light chains) antibody conjugated with Horseradish
  • label filter
    • HRP
  • technique filter
    • Antibody
    • light
    • anti
    • Goat
    • IgG
  • Technique
    Antibody, light, anti, Goat, IgG, antibody to, antibodies against human proteins, antibodies for, antibody Conjugates, goats, IgGs
  • Alternative technique
    antibodies, caprine, immunoglobulins