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Goat anti Bovine IgG (heavy and light chains)

  • Catalog number
    GAB/IgG(H+L)
  • Product name
    Goat anti Bovine IgG (heavy and light chains)
  • Size
    1 mL
  • Category
    Secondary Antibodies
  • Long description
    The reactivity of the antiserum is directed to the Fc and Fab subunits of both subclasses, IgG1 and IgG2. It includes a certain degree of reactivity with other immunoglobulins via the common Fab portion. In immunoelectrophoresis against bovine serum a single characteristic precipitin line with a spur is usually obtained. It does not react with any non-Ig protein in bovine serum, as tested by immunoelectrophoresis and double radial immunodiffusion. In immunoelectrophoresis and double radial immunodiffusion to identify the presence of IgG; as secondary antibody to precipitate the immunoglobulin in normal bovine serum; to prepare an immunoadsorbent for the production of Ig-free bovine serum, plasma or non-Ig preparations. This antiserum is not intended for use in non-precipitating antibody-binding or other highly sensitive assays. For such uses labelled and unlabelled cytochemical and immunoassay reagents meeting the necessary additional specificity and performance requirements are available.
  • Antibody come from
    Purified normal IgG isolated from pooled bovine serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
  • Other description
    Delipidated, heat inactivated lyophilized stable whole serum No preservative added, as it may interfere with the antibody activity. No foreign protein added. Total protein and IgG concentration in the antiserum are comparable to those of pooled goat serum. No foreign proteins added.
  • Clone
    Polyclonal
  • Isotype
    not specified, IgG
  • Antigen antibody binding interaction
    Goat anti Bovine IgG (heavy and light chains) Antibody
  • Antibody is raised in
    Goat
  • Antibody s reacts with
    Bovine
  • Antibody s reacts with these species
    Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail, however tested in double radial immunodiffusion no precipitation has been observed with Human and Swine.
  • Label
    unlabeled
  • Antibody s specificity
    Precipitating polyclonal Goat antiSerum to Bovine IgG, heavy and light chains
  • Research interest
    Veterinary
  • Application
    Immunoprecipitation
  • Antibody s suited for
    Immunoprecipitation. In immunoelectrophoresis use 2 μl or equivalent against 120 μl antiserum. In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 μl antiserum in a 3 mm diameter centre well and 2 μl serum samples (neat and diluted) in 2 mm diameter peripheral wells. Precipitin titre not less than 1:128 when tested against normal bovine serum in agar block titration.
  • Storage
    The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. It is reconstituted by adding 1 ml sterile distilled water. Working dilutions are prepared by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product. Lyophilized at +4° C--at least 10 years. Reconstituted at or below -20° C--3-5 years. Reconstituted at +4° C--7 days
  • Relevant references
    no information yet
  • Protein number
    see ncbi
  • Warnings
    This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
  • Antibody s link
    Antibody_s_link
  • Gene target
    heavy light chains
  • Host
    Goat, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial.
  • isotype filter
    • not specified
  • Short name
    Goat anti IgG (heavy light chains)
  • label filter
    • unlabeled
  • technique filter
    • light
    • anti
    • Goat
    • IgG
  • Technique
    light, anti, Goat, IgG, antibody to, goats, IgGs
  • Alternative technique
    antibodies, caprine, immunoglobulins