- Catalog numberR32178
- Product nameRelB Antibody
- Size0.1mg
- PriceAsk For Price
- Short nameAnti-RelB
- CategoryAntibody
- Concentration0.5mg/ml if reconstituted with 0.2ml sterile DI water
- FormAntigen affinity purified
- ConjugationUnconjugated
- ClonePolyclonal antibody
- Recognised antigenRelB
- Host animalRabbit (Oryctolagus cuniculus)
- ClonalityPolyclonal (rabbit origin)
- IsotypeRabbit IgG
- Species reactivityHuman (Homo sapiens) ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
- Tested applicationsWB
- Recommended dilutionsWestern blot: 0.1-0.5ug/ml
- NotesOptimal dilution of the Rel-B antibody should be determined by the researcher.
- Intented useThis Rel-B antibodyis to be used only for research purposes and not for diagnostics..
- UniprotQ01201
- PurityAntigen affinity
- DescriptionRELB (v-rel reticuloendotheliosis viral oncogene homolog B) is also known as IREL. The International Radiation Hybrid Mapping Consortium assigned the RELB gene to chromosome 19. By RT-PCR and immunocytochemical analyses, Clark et al. (1999) showed that RELB expression correlated with dendritic cell activation. NF-kappa-B-inducing kinase is required for osteoclastogenesis in response to pathologic stimuli. Vaira et al. (2008) found that overexpression of Relb, but not Rela, rescued differentiation of mouse Nik -/- osteoclast precursors, indicating that blockade of the alternative NF-kappa-B pathway, rather than the classical NF-kappa-B pathway, is responsible for the osteoclastogenic defect in the absence of Nik. Using Relb -/- mice, they showed that Relb itself was required for Rankl-induced osteoclastogenesis in vitro and for TNF-induced bone resorption in vivo. Both Relb -/- and Nik -/- mice were resistant to tumor-mediated osteolysis. Vaira et al. (2008) concluded that the alternative NF-kappa-B pathway, via RELB, plays an essential and unique role in RANKL signaling toward osteoclast development.
- ImmunogenAmino acids RHSFNNLGIQCVRKKEIEAAIERKIQLGIDPYNA of human RELB were used as the immunogen for the Rel-B antibody.
- StorageAfter reconstitution, the Rel-B antibody may be kept for up to one month refrigerated at +4 degrees C.For long-term, aliquot and store at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized. Avoid repeated freezing and thawing.
- Alternative to gene target
- [ "v-rel reticuloendotheliosis viral oncogene homolog B", "I-REL and IREL and REL-B", "RELB and IDBG-57021 and ENSG00000104856 and 5971", "protein binding", "nuclei", "Relb and IDBG-151419 and ENSMUSG00000002983 and 19698", "RELB and IDBG-639330 and ENSBTAG00000038428 and 522670" ]
- Gene targetRelB
- Identity:HGNC:9956
- Gene:RELB
- Long gene name:RELB proto-oncogene, NF-kB subunit
- Synonyms gene name:v-rel avian reticuloendotheliosis viral oncogene homolog B (nuclear factor of kappa light polypeptide gene enhancer in B-cells 3)
- Synonyms:REL-B
- Discovery year:1995-10-02
- Entrez gene record:5971
- Pubmed identification:1531086
- Classification:IPT domain containing, NF-kappa B complex subunits
- VEGA ID:OTTHUMG00000162116
- Gene symbolRELB
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- Rabbit IgG
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- Antibody
- TechniqueAntibody, antibodies against human proteins, antibodies for
- Alternative techniqueantibodies