RelB Antibody

• Catalog number
  R30901
• Price
  Please ask
• Size
  0.1mg

• Category
  Antibody
• Concentration
  0.5mg/ml if reconstituted with 0.2ml sterile DI water
• Form
  Antigen affinity purified
• Conjugation
  Unconjugated
• Clone
  Polyclonal antibody
• Recognised antigen
  RelB
• Host animal
  Rabbit (Oryctolagus cuniculus)
• Clonality
  Polyclonal (rabbit origin)
• Species reactivity
  Human (Homo sapiens), Mouse (Mus musculus), Rat ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
• Tested applications
  WB
• Recommended dilutions
  Western blot: 0.5-1ug/ml
• Notes
  The stated application concentrations are suggested starting amounts. Titration of the RelB antibody may be required due to differences in protocols and secondary/substrate sensitivity.
• Intented use
  This RelB antibodyis to be used only for research purposes and not for diagnostics..
• Uniprot
  Q01201
• Purity
  Antigen affinity
• Description
  RelB (v-rel reticuloendotheliosis viral oncogene homolog B) is also known as IREL. By RT-PCR and immunocytochemical analyses, Clark et al.(1999) showed that protein expression correlated with dendritic cell activation. NF-kappa-B-inducing kinase is required for osteoclastogenesis in response to pathologic stimuli. Vaira et al.(2008) found that overexpression of RelB, but not RelA, rescued differentiation of mouse Nik -/- osteoclast precursors, indicating that blockade of the alternative NF-kappa-B pathway, rather than the classic pathway, is responsible for the osteoclastogenic defect in the absence of Nik. Using RelB -/- mice, they showed that the protein itself was required for RankL-induced osteoclastogenesis in vitro and for TNF-induced bone resorption in vivo. Both RelB -/- and Nik -/- mice were resistant to tumor-mediated osteolysis. Vaira et al.(2008) concluded that the alternative NF-kappa-B pathway, via RelB, plays an essential and unique role in RankL signaling toward osteoclast development.
• Immunogen
  Amino acids 393-413 (LPFTYLPRDHDSYGVDKKRKR-human) were used as the immunogen for this RelB antibody (100% mouse homology).
• Storage
  After reconstitution, the Rel B antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized. Avoid repeated freezing and thawing.
• Properties
  If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  RelB  
• Gene symbol
  RELB
• Short name
  Anti-RelB
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Rabbit IgG
• Alternative name
  Antibodies to RelB
• Alternative technique
  antibodies
• Alternative to gene target
  v-rel reticuloendotheliosis viral oncogene homolog B, I-REL and IREL and REL-B, RELB and IDBG-57021 and ENSG00000104856 and 5971, protein binding, nuclei, Relb and IDBG-151419 and ENSMUSG00000002983 and 19698, RELB and IDBG-639330 and ENSBTAG00000038428 and 522670

Gene info

• Identity
  HGNC:9956
• Gene
  RELB
• Long gene name
  RELB proto-oncogene, NF-kB subunit
• Synonyms gene name
  • v-rel avian reticuloendotheliosis viral oncogene homolog B (nuclear factor of kappa light polypeptide gene enhancer in B-cells 3)
• Synonyms
  • REL-B
• GenBank acession
  • M83221
• Locus
  19q13.32
• Discovery year
  1995-10-02
• Entrez gene record
  5971
• Pubmed identfication
  • 1531086
• Classification
  • IPT domain containing
  • NF-kappa B complex subunits
• VEGA ID
  OTTHUMG00000162116

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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