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NFKB2 Antibody

  • Catalog number
    R31047
  • Product name
    NFKB2 Antibody
  • Size
    0.1mg
  • Price
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  • Contact us
    NFKB2 Antibody
  • Short name
    Anti-NFKB2
  • Category
    Antibody
  • Concentration
    0.5mg/ml if reconstituted with 0.2ml sterile DI water
  • Form
    Antigen affinity purified
  • Conjugation
    Unconjugated
  • Clone
    Polyclonal antibody
  • Recognised antigen
    NFKB2
  • Host animal
    Rabbit (Oryctolagus cuniculus)
  • Clonality
    Polyclonal (rabbit origin)
  • Isotype
    Rabbit IgG
  • Species reactivity
    Mouse (Mus musculus), Rat ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
  • Tested applications
    WB, IHC-P
  • Recommended dilutions
    Western blot: 0.5-1ug/ml,IHC (Paraffin): 0.5-1ug/ml
  • Notes
    The stated application concentrations are suggested starting amounts. Titration of the NFKB2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
  • Intented use
    This NFKB2 antibodyis to be used only for research purposes and not for diagnostics..
  • Uniprot
    Q9WTK5
  • Purity
    Antigen affinity
  • Description
    Nuclear Factor Kappa-B, Subunit 2, also known as NFKB or p52/p100 subunit, is a protein that in humans is encoded by the NFKB2 gene. Liptay et al.(1992) mapped the gene for what they called the p49/p100 subunit of NFKB to chromosome 10 by Southern blot analysis of panels of human/Chinese hamster cell hybrids. By fluorescence in situ hybridization(FISH), they confirmed the localization and mapped the gene with greater resolution to 10q24. NFKB2 appears to be the same as LYT10. Claudio et al.(2002) showed that bone marrow(BM) cells from Nfkb2-deficient mice, but not Nfkb1-deficient mice, failed to increase relative and total IgD-positive transitional-1(T1) stage B cells in response to Baff. In vivo, however, Nfkb2-deficient mice did generate mature B cells, but at reduced numbers. Mice of the aly/aly strain, which are naturally deficient in Nik, and mice of the A/WySNJ strain, which have a mutation in Baffr, also failed to produce T1 B cells in response to Baff. Baff stimulation enhanced expression of Bcl2 in T1 B cells, thereby promoting B-cell survival, and caused the processing of the p100 form of the protein to p52, which again required Baffr and Nik, but not Nemo(IKKG). Immunoblot analysis showed that BM cells contained primarily p100.
  • Immunogen
    An amino acid sequence from the N-terminus of mouse NFKB2 (YDPGLDGIPEYDDFEFS) was used as the immunogen for this NFKB2 (100% rat homology).
  • Storage
    After reconstitution, the NFKB2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized. Avoid repeated freezing and thawing.
  • Alternative to gene target
    • [ "nuclear factor of kappa light polypeptide gene enhancer in B-cells 2 (p49/p100)", "CVID10 and H2TF1 and LYT-10 and LYT10 and NF-kB2 and p100 and p52", "NFKB2 and IDBG-87893 and ENSG00000077150 and 4791", "protein binding", "nuclei", "Nfkb2 and IDBG-172544 and ENSMUSG00000025225 and 18034", "BT.20288 and IDBG-639157 and ENSBTAG00000006017 and 526392" ]
  • Gene target
    NFKB2
    • Gene info
    Locus Specific Databases:LRG_1347
  • Gene symbol
    NFKB2
  • isotype filter
    • Rabbit IgG
  • technique filter
    • Antibody
  • Technique
    Antibody, antibodies against human proteins, antibodies for
  • Alternative technique
    antibodies