- Catalog numberF40005-0.08ML
- Product nameJMJD4 Antibody
- Size0.08 ml
- PriceAsk For Price
- Short nameAnti-JMJD4
- CategoryAntibody
- ConcentrationIn 1X PBS, pH 7.4, with 0.09% sodium azide
- FormPurified
- ConjugationUnconjugated
- ClonePolyclonal antibody
- Recognised antigenJMJD4
- Host animalRabbit (Oryctolagus cuniculus)
- ClonalityPolyclonal (rabbit origin)
- IsotypeRabbit Ig
- Species reactivityHuman (Homo sapiens) ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
- Tested applicationsWB, ELISA
- Recommended dilutionsWestern blot: 1:1000
- NotesTitration of the JMJD4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
- Intented useThis JMJD4 antibodyis to be used only for research purposes and not for diagnostics..
- UniprotQ9H9V9
- PurityPurified
- DescriptionCovalent modification of histones plays critical role in regulating chromatin structure and transcription. While most covalent histone modifications are reversible, only recently has it been established that methyl groups are subject to enzymatic removal from histones. A family of novel JmjC domain-containing histone demethylation (JHDM) enzymes have been identified that perform this specific function. Histone demethylation by JHDM proteins requires cofactors Fe(II) and alpha-ketoglutarate. Family members include JHDM1 (demethylating histone 3 at lysine 36), and JHDM2A as well as JMJD2CH3K9 (both of which demethylate histone 3 at lysine 9). Contributions of histone demethylase activity to tumor development, decreases in cell proliferation, and hormone-dependent transcriptional activation have been observed.
- ImmunogenA portion of amino acids 1-30 from the human protein was used as the immunogen for this JMJD4 antibody.
- StorageAliquot the JMJD4 antibody and store frozen at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized.
- Gene targetJMJD4
- Identity:HGNC:25724
- Gene:JMJD4
- Long gene name:jumonji domain containing 4
- Synonyms:FLJ12517
- Discovery year:2005-05-18
- Entrez gene record:65094
- Pubmed identification:24486019
- RefSeq identity:NM_023007
- Classification:JmjC hydroxylases
- VEGA ID:OTTHUMG00000037698
- Gene symbolJMJD4
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- Rabbit Ig
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- Antibody
- TechniqueAntibody, antibodies against human proteins, antibodies for
- Alternative techniqueantibodies