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GCN2 Antibody

  • Catalog number
    R31137
  • Product name
    GCN2 Antibody
  • Size
    0.1mg
  • Price
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    GCN2 Antibody
  • Short name
    Anti-GCN2
  • Category
    Antibody
  • Concentration
    0.5mg/ml if reconstituted with 0.2ml sterile DI water
  • Form
    Antigen affinity purified
  • Conjugation
    Unconjugated
  • Clone
    Polyclonal antibody
  • Recognised antigen
    GCN2
  • Host animal
    Rabbit (Oryctolagus cuniculus)
  • Clonality
    Polyclonal (rabbit origin)
  • Isotype
    Rabbit IgG
  • Species reactivity
    Mouse (Mus musculus), Rat ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
  • Tested applications
    WB
  • Recommended dilutions
    Western blot: 0.5-1ug/ml
  • Notes
    The stated application concentrations are suggested starting amounts. Titration of the GCN2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
  • Intented use
    This GCN2 antibodyis to be used only for research purposes and not for diagnostics..
  • Uniprot
    Q9QZ05
  • Purity
    Antigen affinity
  • Description
    Eukaryotic Translation Initiation Factor 2-Alpha Kinase 4, also called GCN2 (General control nonderepressible 2), is an enzyme that in humans is encoded by the EIF2AK4 gene. It belongs to a family of kinases that phosphorylate the alpha subunit of eukaryotic translation initiation factor-2 to downregulate protein synthesis in response to varied cellular stresses. Hartz(2005) mapped the gene to chromosome 15q15.1 based on an alignment of the protein sequence with the genomic sequence. Berlanga et al.(1999) demonstrated that GCN2 immunopurified from mouse liver extracts could phosphorylate rabbit Eif2 in vitro. Serum starvation increased the level of phosphorylated EIF2-alpha more than 2-fold in human embryonic kidney cells transfected with mouse Eif2ak4. Costa-Mattioli et al.(2005) reported a unique feature of hippocampal slices from GCN2-null mice: in CA1, a single 100-Hz train induced a strong and sustained long-term potentiation(late LTP or L-LTP), which was dependent on transcription and translation.
  • Immunogen
    An amino acid sequence from the middle region of mouse GCN2 (ATDHLAFTAEGKQDDQAGD) was used as the immunogen for this GCN2 antibody.
  • Storage
    After reconstitution, the GCN2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized. Avoid repeated freezing and thawing.
  • Gene target
    GCN2
    • Gene info
  • Gene symbol
    EIF2AK4
  • isotype filter
    • Rabbit IgG
  • technique filter
    • Antibody
  • Technique
    Antibody, antibodies against human proteins, antibodies for
  • Alternative technique
    antibodies