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Gapdh Antibody (Zebrafish)

  • Catalog number
    F52573-0.08ML
  • Product name
    Gapdh Antibody (Zebrafish)
  • Size
    0.08 ml
  • Short name
    Anti-Gapdh (Zebrafish)
  • Category
    Antibody
  • Concentration
    In 1X PBS pH 7.4 with 0.09% sodium azide
  • Form
    Antigen affinity purified
  • Conjugation
    Unconjugated
  • Clone
    Polyclonal antibody
  • Recognised antigen
    Gapdh (Zebrafish)
  • Host animal
    Rabbit (Oryctolagus cuniculus)
  • Clonality
    Polyclonal (rabbit origin)
  • Isotype
    Rabbit Ig
  • Species reactivity
    Human (Homo sapiens), Zebrafish ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
  • Tested applications
    WB, ELISA
  • Recommended dilutions
    Western blot: 1:1000
  • Notes
    Titration of the Zebrafish Gapdh antibody may be required due to differences in protocols and secondary/substrate sensitivity.
  • Intented use
    This Zebrafish Gapdh antibodyis to be used only for research purposes and not for diagnostics..
  • Uniprot
    Q5XJ10
  • Purity
    Antigen affinity
  • Description
    Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3- phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Modulates the organization and assembly of the cytoskeleton. Also participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S- nitrosylation of nuclear target proteins (By similarity).
  • Immunogen
    This Gapdh antibody was produced from a rabbit immunized with a KLH conjugated synthetic peptide between 273-298 amino acids from the C-terminal region of zebrafish Gapdh.
  • Storage
    Aliquot the Zebrafish Gapdh antibody and store frozen at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized.
  • Gene target
    Gapdh Zebrafish
  • isotype filter
    • Rabbit Ig
  • technique filter
    • Antibody
  • Technique
    Antibody, antibodies against human proteins, antibodies for
  • Alternative technique
    antibodies