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Flt3 ligand Antibody

  • Catalog number
    R31014
  • Product name
    Flt3 ligand Antibody
  • Size
    0.1mg
  • Short name
    Anti-Flt3 ligand
  • Category
    Antibody
  • Concentration
    0.5mg/ml if reconstituted with 0.2ml sterile DI water
  • Form
    Antigen affinity purified
  • Conjugation
    Unconjugated
  • Clone
    Polyclonal antibody
  • Recognised antigen
    Flt3 ligand
  • Host animal
    Rabbit (Oryctolagus cuniculus)
  • Clonality
    Polyclonal (rabbit origin)
  • Isotype
    Rabbit IgG
  • Species reactivity
    Human (Homo sapiens) ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
  • Tested applications
    WB
  • Recommended dilutions
    Western blot: 0.5-1ug/ml
  • Notes
    The stated application concentrations are suggested starting amounts. Titration of the Flt3 ligand antibody may be required due to differences in protocols and secondary/substrate sensitivity.
  • Intented use
    This Flt3 ligand antibodyis to be used only for research purposes and not for diagnostics..
  • Uniprot
    P49771
  • Purity
    Antigen affinity
  • Description
    Flt3 (FMS-Related Tyrosine Kinase 3) Ligand, also called FL or FLT3L, is a protein which in humans is encoded by the FLT3LG gene. The protein controls the development of DCs and is particularly important for plasmacytoid DCs and CD8-positive classical DCs and their CD103-positive tissue counterparts. FLT3L is a hematopoietic four helical bundlecytokine. It is structurally homologous to stem cell factor (SCF) and colony stimulating facor 1 (CSF-1). In synergy with other growth factors, FLT3L stimulates the proliferation and differentiation of various blood cell progenitors. Lyman et al.(1993) found that it stimulated proliferation of hematopoietic progenitor cells isolated from mouse fetal liver or adult mouse bone marrow. Hannum et al.(1994) concluded that FLT3L enhances the response of stem and primitive progenitor cells to other growth factors to generate all myeloid lineages except erythroid cells. Assays of C-terminally truncated FL proteins confirmed that the N-terminal half conferred FL activity. Depletion of the Pi3k -Mtor negative regulator Pten facilitated FLT3L-driven DC development in culture.
  • Immunogen
    An amino acid sequence from the C-terminus of human Flt3 ligand (HWQRTRRRTPRPGEQ) was used as the immunogen for this Flt3 ligand antibody.
  • Storage
    After reconstitution, the Flt3 ligand antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized. Avoid repeated freezing and thawing.
  • Alternative to gene target
    • [ "fms-related tyrosine kinase 3", "CD135 and FLK-2 and FLK2 and STK1", "FLT3 and IDBG-19891 and ENSG00000122025 and 2322", "transferase activity", "nuclei", "Flt3 and IDBG-209045 and ENSMUSG00000042817 and 14255", "BT.28173 and IDBG-630678 and ENSBTAG00000018572 and 512700" ]
  • Gene target
    Flt3 ligand
  • isotype filter
    • Rabbit IgG
  • technique filter
    • Antibody
  • Technique
    Antibody, antibodies against human proteins, antibodies for
  • Alternative technique
    antibodies