- Catalog numberR32615
- Product nameAPRT Antibody
- Size0.1mg
- PriceAsk For Price
- Short nameAnti- APRT
- CategoryAntibody
- Concentration0.5mg/ml if reconstituted with 0.2ml sterile DI water
- FormAntigen affinity purified
- ConjugationUnconjugated
- ClonePolyclonal antibody
- Recognised antigenAPRT
- Host animalRabbit (Oryctolagus cuniculus)
- ClonalityPolyclonal (rabbit origin)
- IsotypeRabbit IgG
- Species reactivityHuman (Homo sapiens) ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
- Tested applicationsWB
- Recommended dilutionsWestern blot: 0.5-1ug/ml
- NotesOptimal dilution of the APRT antibody should be determined by the researcher.
- Added bufferLyophilized from 1X Phosphate Buffered Saline (PBS) with 2.5% BSA and 0.025% sodium azide
- Intented useThis APRT antibodyis to be used only for research purposes and not for diagnostics..
- UniprotP07741
- PurityAntigen affinity
- DescriptionAdenine phosphoribosyltransferase (APRTase) is an enzyme encoded by the APRT gene, found in humans on chromosome 16. It belongs to the purine/pyrimidine phosphoribosyltransferase family. A conserved feature of this gene is the distribution of CpG dinucleotides. This enzyme catalyzes the formation of AMP and inorganic pyrophosphate from adenine and 5-phosphoribosyl-1-pyrophosphate (PRPP). It also produces adenine as a by-product of the polyamine biosynthesis pathway. A homozygous deficiency in this enzyme causes 2,8-dihydroxyadenine urolithiasis. Two transcript variants encoding different isoforms have been found for this gene.
- ImmunogenAmino acids 5-49 (ELQLVEQRIRSFPDFPTPGVVFRDISPVLKDPASFRAAIGLLARH-human) were used as the immunogen for the APRT antibody.
- StorageAfter reconstitution, the APRT antibody may be kept for up to one month refrigerated at +4 degrees C.For long-term, aliquot and store at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized. Avoid repeated freezing and thawing.
- Gene targetAPRT
- Identity:HGNC:626
- Gene:APRT
- Long gene name:adenine phosphoribosyltransferase
- Discovery year:2001-06-22
- Entrez gene record:353
- RefSeq identity:NM_000485
- VEGA ID:OTTHUMG00000175454
- Gene symbolAPRT
- isotype filter
- Rabbit IgG
- technique filter
- Antibody
- TechniqueAntibody, antibodies against human proteins, antibodies for
- Alternative techniqueantibodies