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Rat Anti-Mouse CD62L/L-selectin-FITC

  • Catalog number
    MBS673416
  • Product name
    Rat Anti-Mouse CD62L/L-selectin-FITC
  • Size
    5x0.5 mg
  • Products type
    Antibody
  • Products short name
    [CD62L (L-Selectin)]
  • Products name syn
    [Rat Anti-Mouse CD62L/L-selectin - FITC; Rat Anti-Mouse CD62L (L-Selectin)]
  • Clonality
    Monoclonal
  • Isotype
    Rat (Fisher) IgG2a kappa
  • Clone
    [MEL-14]
  • Host
    Rat
  • Reactivity
    Mouse
  • Specificity
    CD62L/L-selectin, Mr 90 kDa CD62L, also known as L-selectin, is a type I transmembrane glycoprotein with lectin-like and Epidermal Growth Factor-like domains.^1, 2 It binds to sialylated oligosaccharide determinants on high endothelial venules (HEV) in peripheral lymph nodes.^1, 2 In the mouse, CD62L is expressed on most thymocytes and on peripheral leukocytes, including B and T lymphocytes, neutrophils, monocytes and eosinophils.^3-7 This member of the selectin family of cell adhesion molecules appears to be required for lymphocyte homing to peripheral lymph nodes and for leukocyte extravasation at sites of inflammation.^1, 2, 7-9 Expression of CD62L on lymphocytes and neutrophils is rapidly down-regulated upon cell activation and the level of CD62L expression, along with other markers, distinguishes naive T cells from effector/memory T cells.^10-13 The monoclonal antibody MEL-14 blocks in vitro binding of lymphocytes to peripheral lymph node HEV and inhibits in vivo lymphocyte extravasation into peripheral lymph nodes and the late stages of leukocyte rolling.^1, 2, 7-9
  • Form
    Fluorescein (FITC) Conjugate
  • Storage stability
    The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2-8 degree C. The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C. The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C. The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL or 0.2 mg in 2.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze! The allophycocyanin (APC) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze! The Spectral Red (SPRD), R-phycoerythrin-Cyanine 7 (R-PE-CY 7), and allophycocyanin-Cyanine 7 (APCCY 7) conjugates are supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze! The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below -20 degree C. Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide-free product at -20 degree C immediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored as directed.
  • Tested application
    Flow Cytometry; Immunohistochemistry (Frozen Sections); Immunoprecipitation
  • Gene target
    CD62L/L-selectin
    • Gene info
  • Gene symbol
    SELL
  • isotype filter
    • Rat Fisher IgG2a kappa
  • Short name
    Anti-Mouse CD62L/L-selectin-FITC
  • Label
    FITC
  • label filter
    • FITC
  • technique filter
    • anti-
    • Mouse
    • anti
    • FITC
  • Technique
    anti-, Mouse, anti, FITC, antibody to, antibodies, Fluorescein, mouses
  • Alternative technique
    murine, antibodies, fluorescine