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Rat Anti-Mouse CD25/IL-2Ra-PE/CY7

  • Catalog number
    MBS673296
  • Product name
    Rat Anti-Mouse CD25/IL-2Ra-PE/CY7
  • Size
    0.1 mg
  • Products type
    Antibody
  • Products short name
    [CD25/IL-2R alpha]
  • Products name syn
    [Rat Anti-Mouse CD25/IL-2Ra - Phycoerythrin-Labeled/CY7; Rat Anti-Mouse CD25/IL-2R alpha]
  • Clonality
    Monoclonal
  • Isotype
    Rat IgG2b kappa
  • Clone
    [3C7]
  • Host
    Rat
  • Reactivity
    Mouse
  • Specificity
    Low affinity alpha chain IL-2 receptor, Mr 55 kDa (IL-2R alpha) The IL-2 receptor (IL-2R) exists in three alternative forms made up from the individual components of CD25, CD122, and CD132. CD25 represents the low affinity alpha chain of the IL-2R, a type I transmembrane glycoprotein containing two CCP domains. It is rich in O-linked carbohydrates and has a short cytoplasmic tail.^14 CD25 is expressed on activated T cells, B cells, NK cells and monocytes of all mouse strains tested.^2, 3, 15 Expression of CD25 on activated T lymphocytes is transitory and endogenously regulated.^4 CD25 is expressed on precursor B cells in bone marrow. Its expression is initiated by functional rearrangement and expression of IgM heavy chain genes and is down-regulated when immature B cells mature and express IgD.^5 It is expressed at a higher level on CD4^+CD8^+ thymocytes.^5, 6 It is also expressed on cultured epidermal Langerhans cells.^7 The biochemical features of murine CD25 have been characterized in detail.^8-10 The 3C7 monoclonal antibody reacts with an epitope of CD25 which is distinct from that recognized by clone 7D4.^2, 11, 12 3C7, used in combination with 7D4 in culture, results in higher levels of inhibition of proliferation driven by IL-2 and generation of alloreactive CTL than either of these monoclonal antibodies alone.^2, 13
  • Form
    R - phycoerythrin - Cyanine 7 (R - PE - CY7) Conjugate
  • Storage stability
    The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2- 8 degree C. The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C. The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C. The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL or 0.2 mg in 2.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze! The allophycocyanin (APC) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze! The Spectral Red (SPRD) and R-phycoerythrin-Cyanine 7 (R-PE-CY 7) conjugates are supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze! The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below -20 degree C. Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide-free product at -20 degree C immediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored as directed.
  • Tested application
    Flow Cytometry; Immunoprecipitation
  • Gene target
    CD25/IL-2Ra-PE/
    • Gene info
    Gene info
    Gene info
    Gene info
    Gene info
    Locus Specific Databases:LRG_1248
    Gene info
    Gene info
    Gene info
    Gene info
    Gene info
    Locus Specific Databases:LRG_1223
  • Gene symbol
    ETV3, ERF, IL36G, IL25, IL22, IL18, IL37, IL6R, IL17C, IL17A
  • isotype filter
    • Rat IgG2b kappa
  • Short name
    Anti-Mouse CD25/IL-2Ra-PE/
  • Label
    PE
  • label filter
    • PE
  • technique filter
    • anti-
    • Mouse
    • anti
  • Technique
    anti-, Mouse, anti, antibody to, antibodies, mouses
  • Alternative technique
    murine, antibodies