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Muscarinic Acetylcholine Receptor M2, aa225-359 (Chrm2, CHRM-2, 7TM Receptor, FLJ43243, HM2, MGC120006, MGC120007)

  • Catalog number
    MBS602250
  • Product name
    Muscarinic Acetylcholine Receptor M2, aa225-359 (Chrm2, CHRM-2, 7TM Receptor, FLJ43243, HM2, MGC120006, MGC120007)
  • Size
    0.1 mg
  • Products type
    Antibody
  • Products short name
    [Muscarinic Acetylcholine Receptor M2, aa225-359]
  • Products name syn
    [Anti -Muscarinic Acetylcholine Receptor M2, aa225-359 (Chrm2, CHRM-2, 7TM Receptor, FLJ43243, HM2, MGC120006, MGC120007)]
  • Other names
    [muscarinic acetylcholine receptor M2; Muscarinic acetylcholine receptor M2; muscarinic acetylcholine receptor M2; 7TM receptor; OTTHUMP00000208393; OTTHUMP00000208394; OTTHUMP00000208395; OTTHUMP00000208396; muscarinic M2 receptor; cholinergic receptor, muscarinic 2, isoform a; cholinergic receptor, muscarinic 2]
  • Other gene names
    [CHRM2; CHRM2; HM2; FLJ43243; MGC120006; MGC120007]
  • Clonality
    Monoclonal
  • Isotype
    IgG2a
  • Clone
    [2Q147]
  • Host
    Rat
  • Reactivity
    Human, Monkey, Rat
  • Specificity
    M2 muscarinic acetylcholine receptor. No reactivity with the other subtypes. Species crossreactivity: Human, monkey and rat. Expected to react with most mammalian species.
  • Purity
    Purified Purified immunoglobulin
  • Form
    Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.
  • Storage stability
    May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. IMMUNOHISTOCHEMISTRY PROTOCOL Use on 30 um, free floating, 4% paraformaldehyde fixed rat brain tissue. All steps are performed under constant agitation. 1. 3 x 10 minute washes in TBS (with or without 0.25% Triton). 2. Incubate for 30 minutes in TBS with 3% serum (same as host from secondary antibody). 3. Incubate M9520-13B diluted appropriately in TBS with 1% serum (same as host from secondary antibody) (with or without 0.25% Triton) for 2 hours at RT followed by 16 hours at 4 degree C. 4. 3 x 10 minute washes in TBS. 5. Incubate with secondary antibody diluted appropriately in TBS with 1% serum (same as host from secondary antibody). 6. 3 x 10 minute washes in TBS. 7. ABC Elite (1:200) in TBS. 8. 2 x 10 minute washes in TBS. 9. 1 x 10 minute wash in phosphate buffer (no saline). 10. DAB reaction with 0.06% NiCl added for intensification. 11. 2 x 10 minute washes in PBS. 12. 1 x 10 minute wash in phosphate buffer (no saline).
  • Tested application
    Immunohistochemistry (IHC), Immunocytochemistry (ICC)
  • Gene target
    Muscarinic Acetylcholine Receptor M2 aa225-359 Chrm2 CHRM-2 7TM Receptor FLJ43243 HM2 MGC120006 MGC120007
    • Gene info
    • Identity:HGNC:1951
    • Gene:CHRM2
    • Long gene name:cholinergic receptor muscarinic 2
    • Discovery year:1988-08-04
    • Entrez gene record:1129
    • Classification:Cholinergic receptors muscarinic, MicroRNA protein coding host genes
    • VEGA ID:OTTHUMG00000155658
    Locus Specific Databases:LRG_405
    Gene info
    Gene info
    Gene info
    Gene info
    Gene info
    Gene info
    Gene info
    Gene info
    Gene info
  • Gene symbol
    CHRM2, MIR7-2, MIR329-2, MIR512-2, MIR521-2, MIR1289-2, MIR509-2, KRTAP13-2, RNU6-2, SNORD116-2
  • isotype filter
    • IgG2a
  • Short name
    Muscarinic Acetylcholine Receptor M2, aa225-359 (Chrm2, CHRM-2, 7TM Receptor, FLJ43243, HM2, MGC120006, MGC120007)