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Mouse Anti-Mouse H-2Kd/H-2Dd-BIOT

  • Catalog number
    MBS673686
  • Product name
    Mouse Anti-Mouse H-2Kd/H-2Dd-BIOT
  • Size
    0.5 mg
  • Products type
    Secondary Antibody
  • Products short name
    [Mouse (C3H/HeJ) Anti-Mouse H-2Kd/H-2Dd]
  • Products name syn
    [Mouse Anti-Mouse H-2Kd/H-2Dd - Biotin; Mouse (C3H/HeJ) Anti-Mouse H-2Kd/H-2Dd]
  • Clonality
    Monoclonal
  • Isotype
    Mouse IgG2a kappa
  • Clone
    [34-1-2S]
  • Host
    Mouse
  • Reactivity
    Mouse
  • Specificity
    An epitope in the alpha 3 domain that is common to H-2Kd and H-2Dd The "classical" MHC Class I molecules are histocompatibility antigens encoded by the H-2 gene complex and consist of heterodimers of highly polymorphic alpha chains noncovalently associated with the invariant beta 2-microglobulin.3,4 These antigens are expressed on most nucleated cells but expression varies on different cell types. MHC Class I molecules present endogenously synthesized peptides to CD8+ T lymphocytes, which are usually cytotoxic T cells.5 MHC Class I antigens expressed on thymic epithelial cells regulate the positive and negative selection of CD8+ T cells during T cell ontogeny.3,6 The monoclonal antibody 34-1-2S binds to a common determinant in the alpha 3 domains of H-2Kd and H-2Dd in the presence or absence of beta 2 microglobulin.7,8 It cross reacts with the alpha 3 domain of H-2Kb.1,2
  • Form
    Biotin (BIOT) Conjugate
  • Storage stability
    The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2- 8 degree C. The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C. The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C. The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze! Protect conjugated forms from light. Each reagent is stable for the period shown on the bottle label if stored as directed.
  • Tested application
    Flow Cytometry; Immunohistochemistry (Acetone Fixed/Frozen Sections)
  • Gene target
    H-2Kd/H-2Dd-BIOT
  • isotype filter
    • Mouse IgG2a kappa
  • Short name
    Mouse Anti-Mouse H-2Kd/H-2Dd-BIOT
  • technique filter
    • anti-
    • Mouse
    • anti
  • Technique
    anti-, Mouse, anti, antibody to, antibodies, mouses
  • Alternative technique
    murine, antibodies