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Mouse Anti-Mouse CD45.1/Ly-5.1-FITC

  • Catalog number
    MBS673521
  • Product name
    Mouse Anti-Mouse CD45.1/Ly-5.1-FITC
  • Size
    0.5 mg
  • Products type
    Antibody
  • Products short name
    [CD45.1/Ly-5.1]
  • Products name syn
    [Mouse Anti-Mouse CD45.1/Ly-5.1 - FITC; Mouse Anti-Mouse CD45.1/Ly-5.1]
  • Clonality
    Monoclonal
  • Isotype
    Mouse IgG2a kappa
  • Clone
    [A20]
  • Host
    Mouse
  • Reactivity
    Mouse
  • Specificity
    Mouse CD 45.1 (Leukocyte Common Antigen) The CD45 antigen is an essential regulator of leukocyte activation and development, and is now known to be a protein tyrosine phosphatase, e.g. CD45 is able to dephosphorylate the Src family of protein kinases.1, 2 This activity is now known to be required for signal transduction induced by T and B cell antigen receptor engagement, cytokine signaling and Fc receptor stimulation.1, 2 The functional state of CD45 also has a pronounced effect on lymphocyte development. CD45 exists in multiple forms, such as the restricted variants (CD45R; see companion products 1660, 1665, 1675, 1685 and 1820) and as allelic forms CD45.1/Ly-5.1 and CD45.2/Ly-5.2. The former arises as a result of alternative splicing of different exons. The specificity of antibodies to CD45.1 and CD45.2 has been exploited in studies where resolution of donor and recipient cells is essential, such as in gene therapy or bone marrow transplantation.3, 4 The monoclonal antibody A20 recognizes all mouse leukocytes expressing the CD 45.1 allotype.
  • Form
    Fluorescein (FITC) Conjugate
  • Storage stability
    The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2- 8 degree C. The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C. The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C. The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL or 0.2 mg in 2.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze! The Allophycocyanin (APC) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze! The Spectral Red (SPRD), R-phycoerythrin-Cyanine 5.5 (R-PE-CY 5.5), R-phycoerythrin-Cyanine 7 (R-PE-CY 7), Allophycocyanin-Cyanine 5.5 (APC-CY 5.5) and Allophycocyanin-Cyanine 7 (APCCY 7) conjugates are supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2- 8 degree C. Do not freeze! The Cyanine 5 (CY 5) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C. Protect conjugated forms from light. Each reagent is stable for the period shown on the bottle label if stored as directed.
  • Tested application
    Flow Cytometry; Immunoprecipitation
  • Gene target
    CD45 1/Ly-5 1
    • Gene info
    Gene info
    • Identity:HGNC:9666
    • Gene:PTPRC
    • Long gene name:protein tyrosine phosphatase receptor type C
    • Synonyms:LCAT200GP180
    • Discovery year:1986-01-01
    • Entrez gene record:5788
    • Pubmed identification:2169617
    • RefSeq identity:NM_002838
    • Classification:Protein tyrosine phosphatases receptor type, Fibronectin type III domain containing, CD molecules
    • VEGA ID:OTTHUMG00000035702
    Locus Specific Databases:PTPRCbase: Mutation registry for CD45 deficiencyLRG_95
  • Gene symbol
    PTPRCAP, PTPRC
  • isotype filter
    • Mouse IgG2a kappa
  • Short name
    Mouse Anti-Mouse CD45 1/Ly-5 1-FITC
  • Label
    FITC
  • label filter
    • FITC
  • technique filter
    • anti-
    • Mouse
    • anti
    • FITC
  • Technique
    anti-, Mouse, anti, FITC, antibody to, antibodies, Fluorescein, mouses
  • Alternative technique
    murine, antibodies, fluorescine