Mouse anti Cytokeratin 19 / Keratin 19, conjugated to FITC
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Catalog number
MBS570437
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Price
Please ask
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Size
5x1 mL
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Products_type
Antibody
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Products_short_name
[Cytokeratin 19 / Keratin 19]
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Other_names
[Keratin, type I cytoskeletal 19; Keratin, type I cytoskeletal 19; keratin, type I cytoskeletal 19; keratin 19; Cytokeratin-19; CK-19; Keratin-19; K19]
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Other_gene_names
[KRT19; KRT19; K19; CK19; K1CS; CK-19; K19]
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Clone
[RCK108]
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Reactivity
Human, Zebrafish
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Specificity
RCK108 reacts exclusively with cytokeratin 19 which is present in glandular-type epithelia and most carcinomas. Does not react with hepatocytes and hepatocellular carcinoma.
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Form
Each vial contains 1ml FITC-conjugated anti cytokeratin 19 monoclonal antibody in PBS containing 0.1% BSA, 0.09% sodium azide. Approximately 100 tests.
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Storage_stability
Store at 4 degree C, or in small aliquots at -20 degree C.
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Tested_application
Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
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Properties
This MyBioSource Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MyBioSource FITC is highly sensitive to pH extremes.
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Conjugation
Anti-FITC Antibody
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
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Gene target
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Short name
Mouse anti Cytokeratin 19 / Keratin 19, conjugated FITC
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Technique
Mouse, anti, FITC, antibody to, antibody Conjugates, Fluorescein, mouses
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Host
Mouse
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Isotype
IgG1
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Label
FITC
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Species
Mouse, Mouses
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Alternative name
Mouse antibody to Cytokeratin 19 / Keratin 19, coupled to fluorecein
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Alternative technique
murine, antibodies, fluorescine
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MeSH Data
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Name
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Concept
Scope note:
A method for the study of certain organic compounds within cells, in situ, by measuring the light intensities of the selectively stained areas of cytoplasm. The compounds studied and their locations in the cells are made to fluoresce and are observed under a microscope.
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Tree numbers
- E01.370.225.500.386
- E05.196.712.516.600.240
- E05.200.500.386
- E05.242.386
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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